PMID- 17594458
OWN - NLM
STAT- MEDLINE
DCOM- 20070917
LR  - 20191210
IS  - 0266-8254 (Print)
IS  - 0266-8254 (Linking)
VI  - 45
IP  - 1
DP  - 2007 Jul
TI  - Taguchi approach significantly increases bioremediation process efficiency: a 
      case study with Hg (II) removal by Pseudomonas aeruginosa.
PG  - 36-41
AB  - AIM: Optimization of process parameters for mercury removal by an Hg 
      (II)-reducing Pseudomonas aeruginosa strain. METHODS AND RESULTS: A strain of Ps. 
      aeruginosa was found to reduce 10 mg l(-1) Hg (II) to Hg0 with 70% efficiency in 
      24 h. To optimize process performance, a statistical tool--Taguchi design of 
      experiments (DOE)--was used to carry out 18 well-defined experiments (L18 
      Orthogonal array) with eight variable parameters (viz. agitation, temperature, 
      pH, carbon source, medium volume: flask volume ratio and concentrations of Hg 
      (II), ammonium sulfate and yeast extract). When data obtained were analyzed using 
      specialized software for Taguchi design, Qualitek-4 (Nutek Inc., MI, USA), Hg 
      (II) reduction efficiency was predicted to be 95% in 24 h under the optimized 
      process parameters (also suggested by the software). In the validation 
      experiment, Hg (II) removal of 99.29% in 24 h was indeed obtained. CONCLUSIONS: 
      Using Taguchi DOE, Hg (II) reduction (and hence its removal) using Ps. aeruginosa 
      could be improved by 29.3%. SIGNIFICANCE AND IMPACT OF THE STUDY: Taguchi 
      approach could be employed as an efficient and time-saving strategy for parameter 
      optimization in bioremediation processes.
FAU - Tupe, S G
AU  - Tupe SG
AD  - Agharkar Research Institute, Pune, India.
FAU - Rajwade, J M
AU  - Rajwade JM
FAU - Paknikar, K M
AU  - Paknikar KM
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Lett Appl Microbiol
JT  - Letters in applied microbiology
JID - 8510094
RN  - 0 (Culture Media)
RN  - 0 (Sewage)
RN  - 0 (Water Pollutants, Chemical)
RN  - 53GH7MZT1R (Mercuric Chloride)
SB  - IM
MH  - Biomass
MH  - *Bioreactors
MH  - *Computer Simulation
MH  - Culture Media
MH  - Hydrogen-Ion Concentration
MH  - Mercuric Chloride/*metabolism
MH  - Pseudomonas aeruginosa/growth & development/isolation & purification/*metabolism
MH  - Sewage/microbiology
MH  - *Software
MH  - Temperature
MH  - Water Pollutants, Chemical/metabolism
MH  - Water Purification/*methods
EDAT- 2007/06/28 09:00
MHDA- 2007/09/18 09:00
CRDT- 2007/06/28 09:00
PHST- 2007/06/28 09:00 [pubmed]
PHST- 2007/09/18 09:00 [medline]
PHST- 2007/06/28 09:00 [entrez]
AID - LAM2152 [pii]
AID - 10.1111/j.1472-765X.2007.02152.x [doi]
PST - ppublish
SO  - Lett Appl Microbiol. 2007 Jul;45(1):36-41. doi: 10.1111/j.1472-765X.2007.02152.x.