PMID- 17594940
OWN - NLM
STAT- MEDLINE
DCOM- 20070926
LR  - 20070801
IS  - 1148-5493 (Print)
IS  - 1148-5493 (Linking)
VI  - 18
IP  - 2
DP  - 2007 Jun
TI  - Short-term lipopolysaccharide stimulation induces differentiation of murine bone 
      marrow-derived dendritic cells into a tolerogenic phenotype.
PG  - 78-85
AB  - Dendritic cells (DCs) are professional, antigen-presenting cells, which induce 
      and regulate T cell reactivity. DCs are crucial in innate and adaptive immune 
      responses, and are also involved in central and peripheral tolerance induction. 
      Tolerance can be mediated by immature and semi-mature DCs expressing low levels 
      of co-stimulator and major histocompatibility complex (MHC) molecules. The aim of 
      this study was to investigate the ability of short-term lipopolysaccharide (LPS) 
      stimulation to modulate the stage of differentiation of bone marrow-derived DCs. 
      For this purpose, DCs obtained from DBA1/lacJ mice were stimulated for four 
      (4hLPS/DCs) or 24 (24hLPS/DCs) hours with LPS, using DCs without stimulation 
      (0hLPS/DCs) as a control. Flow cytometry analysis of 4hLPS/DCs showed 
      intermediate CD40 and MHC class II expression, lower than that of 24hLPS/DCs 
      (fully mature), and greater than that of 0hLPS/DCs (immature). A functional assay 
      showed that 4hLPS/DCs displayed increased endocytotic ability compared to 
      24hLPS/DCs, indicating a semi-mature state. 4hLPS/DCs were greater producers of 
      IL-10 protein and TGFbeta1 mRNA than 24hLPS/DCs and immature DCs, displaying a 
      cytokine production pattern that is characteristic of tolerogenic DCs. An assay 
      for antigen-presenting capacity demonstrated that 4hLPS/DCs induced secretion of 
      IL-2 from an OTH4 T cell hybridoma, indicating a functional presenting activity. 
      Finally, the tolerogenic phenotype of 4hLPS/DCs was demonstrated by their ability 
      to interfere with the progression of bovine type II collagen (bII)-induced 
      arthritis (CIA) when they were loaded with bCII antigen and injected into mice 
      with established CIA. We conclude that the stimulation of murine bone 
      marrow-derived DCs with LPS for four hours generates semi-mature DCs with 
      tolerogenic capability.
FAU - Salazar, Lorena
AU  - Salazar L
AD  - Programa Disciplinario de Inmunologia, Instituto de Ciencias Biomedicas (ICBM), 
      Facultad de Medicina, Universidad de Chile, Santiago, Chile.
FAU - Aravena, Octavio
AU  - Aravena O
FAU - Contreras-Levicoy, Juan
AU  - Contreras-Levicoy J
FAU - Pesce, Barbara
AU  - Pesce B
FAU - Catalan, Diego
AU  - Catalan D
FAU - Zuniga, Roberto
AU  - Zuniga R
FAU - Iruretagoyena, Mirentxu
AU  - Iruretagoyena M
FAU - Kalergis, Alexis M
AU  - Kalergis AM
FAU - Aguillon, Juan C
AU  - Aguillon JC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070626
PL  - France
TA  - Eur Cytokine Netw
JT  - European cytokine network
JID - 9100879
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 130068-27-8 (Interleukin-10)
RN  - 187348-17-0 (Interleukin-12)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/*cytology
MH  - Cell Differentiation
MH  - Cell Separation
MH  - Dendritic Cells/*cytology
MH  - Flow Cytometry
MH  - Interleukin-10/metabolism
MH  - Interleukin-12/metabolism
MH  - Lipopolysaccharides/*metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Inbred DBA
MH  - T-Lymphocytes/metabolism
MH  - Transforming Growth Factor beta1/metabolism
EDAT- 2007/06/28 09:00
MHDA- 2007/09/27 09:00
CRDT- 2007/06/28 09:00
PHST- 2007/06/12 00:00 [accepted]
PHST- 2007/06/28 09:00 [pubmed]
PHST- 2007/09/27 09:00 [medline]
PHST- 2007/06/28 09:00 [entrez]
AID - ecn.2007.0089 [pii]
AID - 10.1684/ecn.2007.0089 [doi]
PST - ppublish
SO  - Eur Cytokine Netw. 2007 Jun;18(2):78-85. doi: 10.1684/ecn.2007.0089. Epub 2007 
      Jun 26.