PMID- 17595337
OWN - NLM
STAT- MEDLINE
DCOM- 20071113
LR  - 20181113
IS  - 0022-1554 (Print)
IS  - 1551-5044 (Electronic)
IS  - 0022-1554 (Linking)
VI  - 55
IP  - 11
DP  - 2007 Nov
TI  - Fluorescence in situ hybridization using an old world monkey Y chromosome 
      specific probe combined with immunofluorescence staining on rhesus monkey 
      tissues.
PG  - 1115-21
AB  - To date, there is no commercially available Y chromosome probe that can be used 
      for fluorescence in situ hybridization (FISH) for the male rhesus monkey. We have 
      recently generated a probe for FISH with high specificity to the short arm of the 
      rhesus monkey Y chromosome. In this study, we further describe a method that 
      keeps the integrity of tissue-specific antigenic structures for 
      immunofluorescence staining subsequent to FISH on paraffin-embedded rhesus monkey 
      tissues. We have examined this technique in combination with an epithelial 
      cell-specific marker, cytokeratin 8/18 (CK8/18), on various tissues, including 
      jejunum, liver, kidney, and pancreas. CK8/18 and Y chromosome signals were 
      distinctly seen simultaneously on epithelial cells from the same tissue section 
      from male but not female monkeys. These studies indicate that our FISH 
      immunofluorescence technique can be reliably used to identify and phenotype male 
      cells in paraffin-embedded rhesus monkey tissues.
FAU - Xia, Xiujin
AU  - Xia X
AD  - Department of Pharmacologiy, Tulane University Health Sciences Center, New 
      Orleans, LA, USA.
FAU - Rasmussen, Terri
AU  - Rasmussen T
FAU - Alvarez, Xavier
AU  - Alvarez X
FAU - Taguchi, Takahiro
AU  - Taguchi T
FAU - Li, Marilyn
AU  - Li M
FAU - La Russa, Vincent F
AU  - La Russa VF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070626
PL  - United States
TA  - J Histochem Cytochem
JT  - The journal of histochemistry and cytochemistry : official journal of the 
      Histochemistry Society
JID - 9815334
RN  - 0 (Cadherins)
RN  - 0 (DNA Probes)
RN  - 0 (Keratin-18)
RN  - 0 (Keratin-8)
SB  - IM
MH  - Animals
MH  - Cadherins/metabolism
MH  - Chromosome Painting/*methods
MH  - *DNA Probes
MH  - Female
MH  - Fluorescent Antibody Technique
MH  - In Situ Hybridization, Fluorescence
MH  - Jejunum/metabolism
MH  - Keratin-18/metabolism
MH  - Keratin-8/metabolism
MH  - Kidney/metabolism
MH  - Liver/metabolism
MH  - Macaca mulatta/*genetics
MH  - Male
MH  - Microscopy, Confocal
MH  - Organ Specificity
MH  - Pancreas/metabolism
MH  - Phenotype
MH  - *Y Chromosome/genetics/metabolism
PMC - PMC3957529
EDAT- 2007/06/28 09:00
MHDA- 2007/11/14 09:00
PMCR- 2008/11/01
CRDT- 2007/06/28 09:00
PHST- 2007/06/28 09:00 [pubmed]
PHST- 2007/11/14 09:00 [medline]
PHST- 2007/06/28 09:00 [entrez]
PHST- 2008/11/01 00:00 [pmc-release]
AID - jhc.7A7216.2007 [pii]
AID - 10.1369_jhc.7A7216.2007 [pii]
AID - 10.1369/jhc.7A7216.2007 [doi]
PST - ppublish
SO  - J Histochem Cytochem. 2007 Nov;55(11):1115-21. doi: 10.1369/jhc.7A7216.2007. Epub 
      2007 Jun 26.