PMID- 17596118
OWN - NLM
STAT- MEDLINE
DCOM- 20071126
LR  - 20181201
IS  - 1076-3279 (Print)
IS  - 1076-3279 (Linking)
VI  - 13
IP  - 10
DP  - 2007 Oct
TI  - Selection of common markers for bone marrow stromal cells from various bones 
      using real-time RT-PCR: effects of passage number and donor age.
PG  - 2405-17
AB  - Bone marrow stromal cells (BMSCs) are valuable in tissue engineering and cell 
      therapy, but the quality of the cells is critical for the efficacy of therapy. To 
      test the quality and identity of transplantable cells, we identified the 
      molecular markers that were expressed at higher levels in BMSCs than in 
      fibroblasts. Using numerous BMSC lines from tibia, femur, ilium, and jaw, 
      together with skin and gum fibroblasts, we compared the gene expression profiles 
      of these cells using DNA microarrays and low-density array cards. The 
      differentiation potential of tibia and femur BMSCs was similar to that of iliac 
      BMSCs, and different from jaw BMSCs, but all BMSC lines had many common markers 
      that were expressed at much higher levels in BMSCs than in fibroblasts; several 
      BMSC markers showed discrete expression patterns between jaw and other BMSCs. The 
      common markers are probably useful in routine tests, but their efficacy may 
      depend upon the passage number or donor age. In our study the passage number 
      markedly altered the expression levels of several markers, while donor age had 
      little effect on them. Considering the effects of in vivo location of BMSCs and 
      passage, magnitude of increase in expression levels, and interindividual 
      differences, we identified several reliable markers -- LIF, IGF1, PRG1, MGP, 
      BMP4, CTGF, KCTD12, IGFBP7, TRIB2, and DYNC1I1 -- among many candidates. This 
      marker set may be useful in a routine test for BMSCs in tissue engineering and 
      cell therapy.
FAU - Igarashi, Akira
AU  - Igarashi A
AD  - Department of Dental and Medical Biochemistry, Graduate School of Biomedical 
      Sciences, Hiroshima University, Hiroshima, Japan.
FAU - Segoshi, Kazumi
AU  - Segoshi K
FAU - Sakai, Yuhiro
AU  - Sakai Y
FAU - Pan, Haiou
AU  - Pan H
FAU - Kanawa, Masami
AU  - Kanawa M
FAU - Higashi, Yukihito
AU  - Higashi Y
FAU - Sugiyama, Masaru
AU  - Sugiyama M
FAU - Nakamura, Kozo
AU  - Nakamura K
FAU - Kurihara, Hidemi
AU  - Kurihara H
FAU - Yamaguchi, Satoru
AU  - Yamaguchi S
FAU - Tsuji, Koichiro
AU  - Tsuji K
FAU - Kawamoto, Takeshi
AU  - Kawamoto T
FAU - Kato, Yukio
AU  - Kato Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Tissue Eng
JT  - Tissue engineering
JID - 9505538
RN  - 0 (Biomarkers)
SB  - IM
MH  - Adult
MH  - Aging/*metabolism
MH  - Biomarkers/metabolism
MH  - Bone Marrow Cells/*cytology/*metabolism
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Female
MH  - Gene Expression Profiling/methods
MH  - Humans
MH  - Male
MH  - Mesenchymal Stem Cells/*cytology/*metabolism
MH  - Middle Aged
MH  - Reverse Transcriptase Polymerase Chain Reaction/methods
MH  - Stromal Cells/*cytology/*metabolism
MH  - Tissue Donors
EDAT- 2007/06/29 09:00
MHDA- 2007/12/06 09:00
CRDT- 2007/06/29 09:00
PHST- 2007/06/29 09:00 [pubmed]
PHST- 2007/12/06 09:00 [medline]
PHST- 2007/06/29 09:00 [entrez]
AID - 10.1089/ten.2006.0340 [doi]
PST - ppublish
SO  - Tissue Eng. 2007 Oct;13(10):2405-17. doi: 10.1089/ten.2006.0340.