PMID- 17597311
OWN - NLM
STAT- MEDLINE
DCOM- 20070911
LR  - 20151119
IS  - 0378-4274 (Print)
IS  - 0378-4274 (Linking)
VI  - 171
IP  - 3
DP  - 2007 Jul 10
TI  - Neurotoxicity in murine striatal dopaminergic pathways following long-term 
      application of low doses of permethrin and MPTP.
PG  - 154-61
AB  - The long-term effects of permethrin (PM) and its interaction with 
      1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) on striatal dopaminergic 
      pathways were investigated in C57BL/6 mice. In a 3-month exposure, technical PM 
      (1.5mg/kg) was administered once per week, with MPTP (20mg/kg) given once on 
      either the 7th week or 11th week. In a 6-month exposure, PM (0.8mg/kg or 
      1.5mg/kg) was administered once per week for 26 weeks, with MPTP (20mg/kg) given 
      once, on week 24. Alterations in the expression of tyrosine hydroxylase (TH), 
      dopamine transporter (DAT), and alpha-synuclein proteins were analyzed 1 day 
      after the last PM treatment using western blot assay. PM had no significant 
      effect on striatal dopaminergic pathways by itself, whereas MPTP significantly 
      reduced the expression of TH and DAT proteins. In both exposure paradigms, weekly 
      1.5mg/kg PM treatments antagonized the toxic effect of MPTP on TH and DAT 
      expression (p<0.05). There was no significant alteration of alpha-synuclein 
      expression following any exposure to PM and/or MPTP. [(3)H]Tetrabenazine (TBZ) 
      binding assay for expression of striatal vesicular monoamine transporter (VMAT) 
      showed no effect of PM, but the reduction in this protein caused by MPTP was 
      attenuated by PM, consistent with effects on other dopaminergic biomarkers. The 
      overall findings demonstrate that long-term, low-dose exposure to PM alone did 
      not cause signs of neurotoxicity to striatal dopaminergic neural terminals, or 
      enhance the effects of MPTP. We conclude that under typical use conditions, PM 
      poses little Parkinsonian hazard to humans, including when impregnated into 
      clothing for control of biting flies.
FAU - Kou, Jinghong
AU  - Kou J
AD  - Neurotoxicology Laboratory, Department of Entomology, Virginia Polytechnic 
      Institute and State University, Blacksburg, VA 24061, USA.
FAU - Bloomquist, Jeffrey R
AU  - Bloomquist JR
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20070521
PL  - Netherlands
TA  - Toxicol Lett
JT  - Toxicology letters
JID - 7709027
RN  - 0 (Affinity Labels)
RN  - 0 (Biomarkers)
RN  - 0 (Dopamine Plasma Membrane Transport Proteins)
RN  - 0 (Insecticides)
RN  - 0 (alpha-Synuclein)
RN  - 509F88P9SZ (Permethrin)
RN  - EC 1.14.16.2 (Tyrosine 3-Monooxygenase)
RN  - VTD58H1Z2X (Dopamine)
RN  - Z9O08YRN8O (Tetrabenazine)
SB  - IM
MH  - Affinity Labels
MH  - Animals
MH  - Biomarkers/analysis
MH  - Blotting, Western
MH  - Dopamine/*physiology
MH  - Dopamine Plasma Membrane Transport Proteins/metabolism
MH  - Insecticides/*toxicity
MH  - MPTP Poisoning/*pathology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Neostriatum/*pathology
MH  - Neural Pathways/pathology
MH  - Permethrin/*toxicity
MH  - Tetrabenazine/pharmacokinetics
MH  - Tyrosine 3-Monooxygenase/metabolism
MH  - Up-Regulation/drug effects
MH  - alpha-Synuclein/biosynthesis
EDAT- 2007/06/29 09:00
MHDA- 2007/09/12 09:00
CRDT- 2007/06/29 09:00
PHST- 2007/02/22 00:00 [received]
PHST- 2007/05/10 00:00 [revised]
PHST- 2007/05/10 00:00 [accepted]
PHST- 2007/06/29 09:00 [pubmed]
PHST- 2007/09/12 09:00 [medline]
PHST- 2007/06/29 09:00 [entrez]
AID - S0378-4274(07)00144-0 [pii]
AID - 10.1016/j.toxlet.2007.05.005 [doi]
PST - ppublish
SO  - Toxicol Lett. 2007 Jul 10;171(3):154-61. doi: 10.1016/j.toxlet.2007.05.005. Epub 
      2007 May 21.