PMID- 17600225
OWN - NLM
STAT- MEDLINE
DCOM- 20070906
LR  - 20171116
IS  - 1524-4636 (Electronic)
IS  - 1079-5642 (Linking)
VI  - 27
IP  - 9
DP  - 2007 Sep
TI  - Enhanced levels of oxidized low-density lipoprotein prime monocytes to cytokine 
      overproduction via upregulation of CD14 and toll-like receptor 4 in unstable 
      angina.
PG  - 1991-7
AB  - OBJECTIVES: The purpose of this study was to establish whether oxidized 
      low-density lipoprotein (oxLDL) contributes to cytokine overproduction via 
      upregulation of CD14 and toll-like receptor-4 (TLR-4) expression on circulating 
      monocytes of unstable angina (UA) patients. METHODS AND RESULTS: Expression of 
      CD14 and TLR-4 on circulating monocytes, and the concentration of plasma oxLDL, 
      (interleukin [IL])-6, IL-1 beta, IL-8, tumor necrosis factor (TNF)-alpha, 
      monocyte chemoattractant protein-1 (MCP-1) were measured in 27 control (C) 
      subjects, 29 patients with stable angina (SA), and 27 with UA. CD14 and TLR-4 
      expression on monocytes and circulating IL-6, IL-1 beta, and oxLDL were higher in 
      UA than in SA and C subjects (P<0.001). In in vitro experiments, oxLDL increased 
      CD14 and TLR-4 expression (P<0.001) in control monocytes as well as IL-6, IL-1 
      beta, and at a lower extent TNF-alpha and MCP-1 levels in the supernatant (P from 
      <0.05 to <0.001). The preincubation of sera derived from UA patients but with 
      control monocytes also induced a significant increase of CD14 and TLR-4 
      expression (P<0.001) and of IL-6 and IL-1 beta production (P<0.001) in the 
      supernatant. CONCLUSIONS: In UA patients oxLDL may contribute to monocyte 
      overproduction of some cytokines by upregulating CD14 and TLR-4 expression.
FAU - Pasini, A Fratta
AU  - Pasini AF
AD  - Department of Biomedical and Surgical Sciences, Section of Internal Medicine D, 
      University of Verona, Policlinico G.B. Rossi, Piazzale L.A. Scuro 10, 37134 
      Verona, Italy.
FAU - Anselmi, M
AU  - Anselmi M
FAU - Garbin, U
AU  - Garbin U
FAU - Franchi, E
AU  - Franchi E
FAU - Stranieri, C
AU  - Stranieri C
FAU - Nava, M C
AU  - Nava MC
FAU - Boccioletti, V
AU  - Boccioletti V
FAU - Vassanelli, C
AU  - Vassanelli C
FAU - Cominacini, L
AU  - Cominacini L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070628
PL  - United States
TA  - Arterioscler Thromb Vasc Biol
JT  - Arteriosclerosis, thrombosis, and vascular biology
JID - 9505803
RN  - 0 (Interleukin-1beta)
RN  - 0 (Interleukin-6)
RN  - 0 (Lipopolysaccharide Receptors)
RN  - 0 (Lipoproteins, LDL)
RN  - 0 (TLR4 protein, human)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (oxidized low density lipoprotein)
SB  - IM
MH  - Aged
MH  - Angina, Unstable/blood/*metabolism
MH  - Female
MH  - Humans
MH  - Interleukin-1beta/metabolism
MH  - Interleukin-6/metabolism
MH  - Lipopolysaccharide Receptors/*metabolism
MH  - Lipoproteins, LDL/*physiology
MH  - Male
MH  - Middle Aged
MH  - Monocytes/*metabolism
MH  - Toll-Like Receptor 4/*metabolism
MH  - Up-Regulation
EDAT- 2007/06/30 09:00
MHDA- 2007/09/07 09:00
CRDT- 2007/06/30 09:00
PHST- 2007/06/30 09:00 [pubmed]
PHST- 2007/09/07 09:00 [medline]
PHST- 2007/06/30 09:00 [entrez]
AID - ATVBAHA.107.142695 [pii]
AID - 10.1161/ATVBAHA.107.142695 [doi]
PST - ppublish
SO  - Arterioscler Thromb Vasc Biol. 2007 Sep;27(9):1991-7. doi: 
      10.1161/ATVBAHA.107.142695. Epub 2007 Jun 28.