PMID- 17601673
OWN - NLM
STAT- MEDLINE
DCOM- 20071031
LR  - 20231213
IS  - 0306-4522 (Print)
IS  - 0306-4522 (Linking)
VI  - 147
IP  - 4
DP  - 2007 Jul 29
TI  - Identification of connexin36 in gap junctions between neurons in rodent locus 
      coeruleus.
PG  - 938-56
AB  - Locus coeruleus neurons are strongly coupled during early postnatal development, 
      and it has been proposed that these neurons are linked by extraordinarily 
      abundant gap junctions consisting of connexin32 (Cx32) and connexin26 (Cx26), and 
      that those same connexins abundantly link neurons to astrocytes. Based on the 
      controversial nature of those claims, immunofluorescence imaging and 
      freeze-fracture replica immunogold labeling were used to re-investigate the 
      abundance and connexin composition of neuronal and glial gap junctions in 
      developing and adult rat and mouse locus coeruleus. In early postnatal 
      development, connexin36 (Cx36) and connexin43 (Cx43) immunofluorescent puncta 
      were densely distributed in the locus coeruleus, whereas Cx32 and Cx26 were not 
      detected. By freeze-fracture replica immunogold labeling, Cx36 was found in 
      ultrastructurally-defined neuronal gap junctions, whereas Cx32 and Cx26 were not 
      detected in neurons and only rarely detected in glia. In 28-day postnatal (adult) 
      rat locus coeruleus, immunofluorescence labeling for Cx26 was always co-localized 
      with the glial gap junction marker Cx43; Cx32 was associated with the 
      oligodendrocyte marker 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase); and 
      Cx36 was never co-localized with Cx26, Cx32 or Cx43. Ultrastructurally, Cx36 was 
      localized to gap junctions between neurons, whereas Cx32 was detected only in 
      oligodendrocyte gap junctions; and Cx26 was found only rarely in astrocyte 
      junctions but abundantly in pia mater. Thus, in developing and adult locus 
      coeruleus, neuronal gap junctions contain Cx36 but do not contain detectable Cx32 
      or Cx26, suggesting that the locus coeruleus has the same cell-type specificity 
      of connexin expression as observed ultrastructurally in other regions of the CNS. 
      Moreover, in both developing and adult locus coeruleus, no evidence was found for 
      gap junctions or connexins linking neurons with astrocytes or oligodendrocytes, 
      indicating that neurons in this nucleus are not linked to the pan-glial syncytium 
      by Cx32- or Cx26-containing gap junctions or by abundant free connexons composed 
      of those connexins.
FAU - Rash, J E
AU  - Rash JE
AD  - Department of Biomedical Sciences, Colorado State University, Campus Delivery 
      1617, Fort Collins, CO 80523, USA. john.rash@colostate.edu
FAU - Olson, C O
AU  - Olson CO
FAU - Davidson, K G V
AU  - Davidson KG
FAU - Yasumura, T
AU  - Yasumura T
FAU - Kamasawa, N
AU  - Kamasawa N
FAU - Nagy, J I
AU  - Nagy JI
LA  - eng
GR  - R01 NS044395-05/NS/NINDS NIH HHS/United States
GR  - NS-44395/NS/NINDS NIH HHS/United States
GR  - S10 RR015706-01A1/RR/NCRR NIH HHS/United States
GR  - R01 NS044010-04/NS/NINDS NIH HHS/United States
GR  - R01 NS044395/NS/NINDS NIH HHS/United States
GR  - R01 NS044010/NS/NINDS NIH HHS/United States
GR  - NS-44010/NS/NINDS NIH HHS/United States
GR  - R56 NS044395/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20070702
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - 0 (Connexins)
RN  - 0 (Nerve Tissue Proteins)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Connexins/classification/deficiency/*metabolism
MH  - Freeze Fracturing/methods
MH  - Gap Junctions/*metabolism/ultrastructure
MH  - Locus Coeruleus/*cytology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Microscopy, Electron, Scanning/methods
MH  - Nerve Tissue Proteins/metabolism
MH  - Neurons/*cytology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Rodentia/growth & development/*metabolism
MH  - Gap Junction delta-2 Protein
PMC - PMC2034517
MID - NIHMS28389
EDAT- 2007/07/03 09:00
MHDA- 2007/11/01 09:00
PMCR- 2008/07/29
CRDT- 2007/07/03 09:00
PHST- 2007/01/13 00:00 [received]
PHST- 2007/04/15 00:00 [revised]
PHST- 2007/04/30 00:00 [accepted]
PHST- 2007/07/03 09:00 [pubmed]
PHST- 2007/11/01 09:00 [medline]
PHST- 2007/07/03 09:00 [entrez]
PHST- 2008/07/29 00:00 [pmc-release]
AID - S0306-4522(07)00550-7 [pii]
AID - 10.1016/j.neuroscience.2007.04.061 [doi]
PST - ppublish
SO  - Neuroscience. 2007 Jul 29;147(4):938-56. doi: 10.1016/j.neuroscience.2007.04.061. 
      Epub 2007 Jul 2.