PMID- 17601727
OWN - NLM
STAT- MEDLINE
DCOM- 20080429
LR  - 20080303
IS  - 0960-8524 (Print)
IS  - 0960-8524 (Linking)
VI  - 99
IP  - 7
DP  - 2008 May
TI  - Purification of extracellular acid protease and analysis of fermentation 
      metabolites by Synergistes sp. utilizing proteinaceous solid waste from 
      tanneries.
PG  - 2364-72
AB  - The untanned proteinaceous tannery solid waste, animal fleshing (ANFL), was used 
      as a substrate for acid protease production by Synergistes sp. The strain was 
      isolated from an anaerobic digester used for the treatment of tannery solid waste 
      and was selected for its enhanced protease production at activity 350-420 U/ml. 
      The optimum pH was in the acidic range of 5.5-6.5 and optimum temperature was in 
      mesophilic range of 25-35 degrees C. The sodium dodecyl sulfate-polyacrylamide 
      gel electrophoresis (SDS-PAGE) and the zymogram analyses of the purified protein 
      indicated an estimated molecular mass of 60 kDa. This protease could be 
      classified as aspartic protease based on its inhibition by aspartate type 
      protease inhibitor pepstatin and on non-inhibition by 1,10-phenanthroline, EDTA, 
      EGTA and phenylmethylsulfonyl fluoride. The degradation of ANFL was confirmed by 
      Gas Chromatography-Mass Spectroscopy (GC-MS), Proton Nuclear Magnetic Resonance 
      Spectroscopy (H1 NMR) and Scanning Electron Microscopy (SEM) analyses. In this 
      study we found that the activity of acid protease depended on factors such as 
      calcium concentration, pH and temperature. Based on these lines of evidence, we 
      postulate that this protease is a highly catalytic novel protease of its type.
FAU - Kumar, A Ganesh
AU  - Kumar AG
AD  - Department of Environmental Technology, Central Leather Research Institute, 
      Adyar, Chennai 600 020, Tamil Nadu, India. microganesh@yahoo.com 
      <microganesh@yahoo.com>
FAU - Nagesh, N
AU  - Nagesh N
FAU - Prabhakar, T G
AU  - Prabhakar TG
FAU - Sekaran, G
AU  - Sekaran G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070629
PL  - England
TA  - Bioresour Technol
JT  - Bioresource technology
JID - 9889523
RN  - 0 (DNA Primers)
SB  - IM
MH  - Bacteria/*metabolism
MH  - Base Sequence
MH  - DNA Primers
MH  - Electrophoresis, Polyacrylamide Gel
MH  - *Fermentation
MH  - Gas Chromatography-Mass Spectrometry
MH  - Hydrogen-Ion Concentration
MH  - Microscopy, Electron, Scanning
MH  - Particle Size
MH  - *Tanning
EDAT- 2007/07/03 09:00
MHDA- 2008/04/30 09:00
CRDT- 2007/07/03 09:00
PHST- 2007/02/12 00:00 [received]
PHST- 2007/05/04 00:00 [revised]
PHST- 2007/05/05 00:00 [accepted]
PHST- 2007/07/03 09:00 [pubmed]
PHST- 2008/04/30 09:00 [medline]
PHST- 2007/07/03 09:00 [entrez]
AID - S0960-8524(07)00415-4 [pii]
AID - 10.1016/j.biortech.2007.05.001 [doi]
PST - ppublish
SO  - Bioresour Technol. 2008 May;99(7):2364-72. doi: 10.1016/j.biortech.2007.05.001. 
      Epub 2007 Jun 29.