PMID- 17602444
OWN - NLM
STAT- MEDLINE
DCOM- 20080117
LR  - 20191210
IS  - 0197-3851 (Print)
IS  - 0197-3851 (Linking)
VI  - 27
IP  - 10
DP  - 2007 Oct
TI  - Fully automated FISH examination of amniotic fluid cells.
PG  - 951-5
AB  - OBJECTIVE: Fluorescence in situ hybridization (FISH) analysis has become a 
      valuable adjunct in cytogenetics, providing a rapid screen for common chromosome 
      abnormalities that is particularly helpful in prenatal diagnosis. FISH analysis 
      using standard microscopy is expensive and labor intensive, requiring both a high 
      skill level and subjective signal interpretation. A reliable fully automated 
      system for FISH analysis could improve laboratory efficiency and potentially 
      reduce errors and costs. METHODS: The efficacy of an automated system was 
      compared to standard manual FISH analysis. Two sets of slides were generated from 
      each of 152 amniotic fluid samples. Following hybridization with a standard panel 
      of five chromosome FISH probes, one set of slides was evaluated using manual 
      microscopy. The other set was evaluated using an automated microscopy system. 
      RESULTS: A diagnostic outcome was obtained for all 152 samples using manual 
      microscopy and for 146 of 152 (96%) samples using automated microscopy. Three 
      cases of aneuploidy were detected. For those samples for which a diagnostic 
      outcome was determined by both manual and automated microscopy, 100% concordance 
      was observed. All FISH analysis results were confirmed by karyotype. CONCLUSION: 
      These data suggest that an automated microscopy system is capable of providing 
      accurate and rapid enumeration of FISH signals in amniocytes.
FAU - Wauters, Jan
AU  - Wauters J
AD  - Department of Genetics, University of Antwerp, Antwerp, Belgium. 
      jan.wauters@ua.ac.be
FAU - Assche, Elvire Van
AU  - Assche EV
FAU - Antsaklis, Aristidis
AU  - Antsaklis A
FAU - Tepperberg, James
AU  - Tepperberg J
FAU - Sharp, S Michael
AU  - Sharp SM
FAU - Kilpatrick, Michael W
AU  - Kilpatrick MW
FAU - Tafas, Triantafyllos
AU  - Tafas T
FAU - Tsipouras, Petros
AU  - Tsipouras P
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PL  - England
TA  - Prenat Diagn
JT  - Prenatal diagnosis
JID - 8106540
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Amniotic Fluid/*cytology
MH  - Automation
MH  - Chromosome Disorders/*diagnosis
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Middle Aged
MH  - Pregnancy
MH  - *Prenatal Diagnosis
MH  - Reproducibility of Results
EDAT- 2007/07/03 09:00
MHDA- 2008/01/18 09:00
CRDT- 2007/07/03 09:00
PHST- 2007/07/03 09:00 [pubmed]
PHST- 2008/01/18 09:00 [medline]
PHST- 2007/07/03 09:00 [entrez]
AID - 10.1002/pd.1806 [doi]
PST - ppublish
SO  - Prenat Diagn. 2007 Oct;27(10):951-5. doi: 10.1002/pd.1806.