PMID- 17605862
OWN - NLM
STAT- MEDLINE
DCOM- 20091211
LR  - 20181201
IS  - 1009-2137 (Print)
IS  - 1009-2137 (Linking)
VI  - 15
IP  - 3
DP  - 2007 Jun
TI  - [Enrichment and biological characteristics of murine mesenchymal stem cells].
PG  - 542-6
AB  - The study was aimed to isolate and establish mesenchymal stem cell line from 
      adult murine bone marrow as well as to identify its biological characteristics 
      and differentiation potential. Bone marrow cells (BMCs) were collected by 
      flushing the femurs and tibias of 4 - 5-week-old male C57BL/6 mice, and were 
      inoculated at a concentration of 1 x 10(6)/cm(2). mMSCs were isolated, enriched 
      and expanded by using bone marrow adherant culture and monoclonal culture. The 
      characteristics of the cells, such as morphology, growth pattern, cell cycle, 
      phenotype, karyotype and multipotent differentiation potential, cytogenetic 
      stability and tumorigenesis were determined. The results indicated that the cell 
      population consisted of spindle- and star-shaped cells, they were highly positive 
      for CD29, CD44, Sca-1, MHC-I, moderate positive for CD13, CD90.2 and negative for 
      CD117, CD45, Flk-1 and MHC-II. mMSCs could be induced to differentiate into 
      adipocytes, osteoblast cells and chondrocytes. It is concluded that mMSC can be 
      isolted, expanded and enriched by using bone marrow adhcrent culture and 
      monoclonal culture. No tumor formations are observed for 3 months in nude mice 
      after subcutaneous injection. mMSCs retain their properties after at least 30 
      passages in culture as well as from frozen stocks.
FAU - Xie, Lin-Na
AU  - Xie LN
AD  - Department of Hematology, Changhai Hospitial, The Second Military Medical 
      University, Shanghai 200433, China.
FAU - Wang, Jian-Min
AU  - Wang JM
FAU - Qiu, Hui-Ying
AU  - Qiu HY
FAU - Gao, Lei
AU  - Gao L
FAU - Zhou, Hong
AU  - Zhou H
FAU - Gong, Sheng-Lan
AU  - Gong SL
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Shi Yan Xue Ye Xue Za Zhi
JT  - Zhongguo shi yan xue ye xue za zhi
JID - 101084424
RN  - 0 (Hyaluronan Receptors)
RN  - 0 (Integrin beta1)
SB  - IM
MH  - Animals
MH  - Cell Differentiation/*physiology
MH  - Cell Proliferation
MH  - Cell Separation/methods
MH  - Cells, Cultured
MH  - Hyaluronan Receptors/metabolism
MH  - Integrin beta1/metabolism
MH  - Male
MH  - Mesenchymal Stem Cells/*cytology/immunology/*physiology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Nude
EDAT- 2007/07/04 09:00
MHDA- 2009/12/16 06:00
CRDT- 2007/07/04 09:00
PHST- 2007/07/04 09:00 [pubmed]
PHST- 2009/12/16 06:00 [medline]
PHST- 2007/07/04 09:00 [entrez]
AID - 1009-2137(2007)03-0542-05 [pii]
PST - ppublish
SO  - Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2007 Jun;15(3):542-6.