PMID- 17605887
OWN - NLM
STAT- MEDLINE
DCOM- 20091211
LR  - 20161018
IS  - 1009-2137 (Print)
IS  - 1009-2137 (Linking)
VI  - 15
IP  - 3
DP  - 2007 Jun
TI  - [Detection of minimal residual disease in children leukemia patients by using PCR 
      - review].
PG  - 652-6
AB  - MRD detection in children leukemia has a potential importance to predict clinical 
      outcome and to modify treatment protocols of the diseases. Although some patients 
      with leukemia have achieved complete remission according to the clinical and 
      morphological criteria, there are still very low numbers of malignant cells that 
      can not be discriminated by morphology and remained in bone marrow, which is 
      called minimal residual disease (MRD) and is the main reason leading to relapse. 
      MRD detection has an important significance for designing treatment protocols. 
      Several methods of MRD detection have been developed. These include conventional 
      cytogenetics, fluorescence in situ hybridization (FISH), flow-cytometric 
      immunophenotyping (FCM), Southern blot and polymerase chain reaction (PCR) 
      techniques, etc. Each of these techniques has its advantages and disadvantages, 
      so not all of them are suitable for clinical MRD detection because of several 
      inherent disadvantages, such as limited sensitivity, time-consuming, high cost, 
      or requiring high-quality DNA or RNA. For example, the sensitivities of 
      conventional cytogenetics, FISH, FCM and Southern blot approaches for MRD 
      monitoring are 10(-1) - 10(-2), 10(-2), 10(-3) - 10(-4) and 10(-1), respectively. 
      Relatively, PCR can reach a good sensitivity of 10(-4) - 10(-6), and show more 
      advantages, such as fast, specific, simple and low-cost, as well as minimal 
      amounts of DNA or RNA for detection, etc., so PCR has its specific features for 
      MRD detection. In this review, the progress on the detection technique for 
      screening leukemia specific marker by muitiplex PCR and FQ-PCR in recent years 
      are summarized.
FAU - He, Ying-Yi
AU  - He YY
AD  - Department of Pediatrics, The First Affiliated Hospital of Guangzhou Medical 
      College, Guangzhou 510120, China.
FAU - Ye, Tie-Zhen
AU  - Ye TZ
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Review
PL  - China
TA  - Zhongguo Shi Yan Xue Ye Xue Za Zhi
JT  - Zhongguo shi yan xue ye xue za zhi
JID - 101084424
SB  - IM
MH  - Child
MH  - Humans
MH  - Leukemia/*diagnosis/genetics
MH  - Neoplasm, Residual/*diagnosis/genetics
MH  - *Polymerase Chain Reaction/methods
MH  - Sensitivity and Specificity
RF  - 15
EDAT- 2007/07/04 09:00
MHDA- 2009/12/16 06:00
CRDT- 2007/07/04 09:00
PHST- 2007/07/04 09:00 [pubmed]
PHST- 2009/12/16 06:00 [medline]
PHST- 2007/07/04 09:00 [entrez]
AID - 1009-2137(2007)03-0652-05 [pii]
PST - ppublish
SO  - Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2007 Jun;15(3):652-6.