PMID- 17606270
OWN - NLM
STAT- MEDLINE
DCOM- 20071203
LR  - 20121115
IS  - 0021-9673 (Print)
IS  - 0021-9673 (Linking)
VI  - 1163
IP  - 1-2
DP  - 2007 Sep 7
TI  - Advances in the o-phthalaldehyde derivatizations. Comeback to the 
      o-phthalaldehyde-ethanethiol reagent.
PG  - 25-42
AB  - The main aims of this work were (a) to present the characteristics and stability 
      of the o-phthalaldehyde (OPA)-ethanethiol (ET) derivatives of 22 amino acids, 
      including the believed-to-be less stable OPA derivatives providing glycine, 
      gamma-aminobutyric acid, beta-alanine, histidine, ornithine, lysine and the 
      C(1)-C(5) aliphatic amines; (b) to compare the stability properties of the most 
      common amino acids and amines as OPA-ET-fluorenylmethyl chloroformate (FMOC) 
      derivatives to the corresponding ones obtained from OPA reagents containing 
      various (SH)-additives; (c) to show the molar responses of alanine and lysine 
      depending on the OPA reagent's composition; as well as (d) to prove the practical 
      utility of these basic researches, by the simultaneous HPLC separation of 22 
      amino acids and 15 amines as their OPA-ET-FMOC derivatives. Investigations have 
      been carried out by varying the composition of the reagents, the molar ratios of 
      reactants and the reaction time, applying diode array and fluorescence detections 
      simultaneously. Average reproducibility of quantitations, characterized with the 
      relative standard deviations (RSDs) based on the fluorescence intensities of 
      derivatives, in the order of listing, proved to be 1.2-5.9% for amino acids and 
      1.1-8.7% for amines. The practical utility of the method is demonstrated by the 
      analysis of the amino acid and amine contents of mouse tissues, with an average 
      reproducibility of 3.5%.
FAU - Hanczko, R
AU  - Hanczko R
AD  - Institute of Chemistry, Department of Analytical Chemistry, L. Eotvos University, 
      PO Box 32, H-1518 Budapest 112, Hungary.
FAU - Jambor, A
AU  - Jambor A
FAU - Perl, A
AU  - Perl A
FAU - Molnar-Perl, I
AU  - Molnar-Perl I
LA  - eng
GR  - R01 DK49221/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Review
DEP - 20070613
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Amino Acids)
RN  - 0 (Indicators and Reagents)
RN  - 0 (Sulfhydryl Compounds)
RN  - 643-79-8 (o-Phthalaldehyde)
RN  - M439R54A1D (ethanethiol)
SB  - IM
MH  - Amino Acids/analysis/chemistry/isolation & purification
MH  - Chromatography, High Pressure Liquid/methods
MH  - Indicators and Reagents/analysis/*chemistry/isolation & purification
MH  - Reproducibility of Results
MH  - Sulfhydryl Compounds/analysis/*chemistry/isolation & purification
MH  - o-Phthalaldehyde/analysis/*chemistry/isolation & purification
RF  - 33
EDAT- 2007/07/04 09:00
MHDA- 2007/12/06 09:00
CRDT- 2007/07/04 09:00
PHST- 2007/01/18 00:00 [received]
PHST- 2007/05/20 00:00 [revised]
PHST- 2007/06/08 00:00 [accepted]
PHST- 2007/07/04 09:00 [pubmed]
PHST- 2007/12/06 09:00 [medline]
PHST- 2007/07/04 09:00 [entrez]
AID - S0021-9673(07)01031-X [pii]
AID - 10.1016/j.chroma.2007.06.013 [doi]
PST - ppublish
SO  - J Chromatogr A. 2007 Sep 7;1163(1-2):25-42. doi: 10.1016/j.chroma.2007.06.013. 
      Epub 2007 Jun 13.