PMID- 17609472
OWN - NLM
STAT- MEDLINE
DCOM- 20071109
LR  - 20131121
IS  - 1525-3163 (Electronic)
IS  - 0021-8812 (Linking)
VI  - 85
IP  - 10
DP  - 2007 Oct
TI  - Gene expression of 3beta-hydroxysteroid dehydrogenase and 17beta-hydroxysteroid 
      dehydrogenase in relation to androstenone, testosterone, and estrone sulphate in 
      gonadally intact male and castrated pigs.
PG  - 2457-63
AB  - Androstenone is one of the main compounds responsible for boar taint, and 
      3beta-hydroxysteroid dehydrogenase (3betaHSD) might be involved in its 
      metabolism. In this study, the gene expression of 3betaHSD and 
      17beta-hydroxysteroid dehydrogenase (17betaHSD) were determined by real-time PCR 
      analysis and related to the concentrations of androstenone, testosterone, and 
      estrone sulphate (E1S). The experiments were performed on gonadally intact male 
      pigs classified based on high or low fat androstenone concentrations, as 
      predetermined by HPLC, as well as on immunocastrated and surgically castrated 
      male pigs. The male pigs with high androstenone concentrations in fat had low 
      3betaHSD gene expression in liver and testis. Moreover, the 17betaHSD gene 
      expression in liver, but not in testis, varied negatively with fat androstenone 
      concentrations. Immunocastrated and surgically castrated male pigs had 
      nondetectable concentrations of fat androstenone and plasma testosterone and E1S, 
      and the castration procedure induced a significant increase of 3betaHSD and 
      17betaHSD gene expression. The mRNA expression was generally much greater from 
      the 3betaHSD than from the 17betaHSD gene. Furthermore, fat androstenone was 
      negatively correlated with liver 3betaHSD gene expression (Pearson correlation, r 
      = -0.69; P < 0.05), and the 17betaHSD gene expression in liver was negatively 
      correlated with plasma E1S (r = -0.95; P < 0.001), indicating an important role 
      of liver 17betaHSD in the estrogen metabolism of gonadally intact male pigs. 
      Another strong correlation was found between 3betaHSD and 17betaHSD gene 
      expression in liver of the gonadally intact male pigs (r = 0.86; P < 0.01), 
      possibly reflecting similar regulation mechanisms of these genes.
FAU - Chen, G
AU  - Chen G
AD  - Department of Food Science, Swedish University of Agricultural Sciences, PO Box 
      7051, SE-750 07 Uppsala, Sweden. Gang.Chen@lmv.slu.se
FAU - Bourneuf, E
AU  - Bourneuf E
FAU - Marklund, S
AU  - Marklund S
FAU - Zamaratskaia, G
AU  - Zamaratskaia G
FAU - Madej, A
AU  - Madej A
FAU - Lundstrom, K
AU  - Lundstrom K
LA  - eng
PT  - Journal Article
DEP - 20070703
PL  - United States
TA  - J Anim Sci
JT  - Journal of animal science
JID - 8003002
RN  - 2DI9HA706A (Estrone)
RN  - 3XMK78S47O (Testosterone)
RN  - C24W7J5D5R (Androsterone)
RN  - EC 1.1.- (17-Hydroxysteroid Dehydrogenases)
RN  - EC 1.1.- (3-Hydroxysteroid Dehydrogenases)
RN  - QTL48N278K (estrone sulfate)
SB  - IM
MH  - 17-Hydroxysteroid Dehydrogenases/*metabolism
MH  - 3-Hydroxysteroid Dehydrogenases/*metabolism
MH  - Androsterone/blood/metabolism
MH  - Animals
MH  - Chromatography, High Pressure Liquid/veterinary
MH  - Estrone/analogs & derivatives/blood/metabolism
MH  - Gene Expression Regulation, Enzymologic
MH  - Liver/enzymology/*metabolism
MH  - Male
MH  - Orchiectomy/*veterinary
MH  - Polymerase Chain Reaction/veterinary
MH  - Swine/*metabolism
MH  - Testis/enzymology/metabolism
MH  - Testosterone/blood/metabolism
EDAT- 2007/07/05 09:00
MHDA- 2007/11/10 09:00
CRDT- 2007/07/05 09:00
PHST- 2007/07/05 09:00 [pubmed]
PHST- 2007/11/10 09:00 [medline]
PHST- 2007/07/05 09:00 [entrez]
AID - jas.2007-0087 [pii]
AID - 10.2527/jas.2007-0087 [doi]
PST - ppublish
SO  - J Anim Sci. 2007 Oct;85(10):2457-63. doi: 10.2527/jas.2007-0087. Epub 2007 Jul 3.