PMID- 17610491
OWN - NLM
STAT- MEDLINE
DCOM- 20070814
LR  - 20070705
IS  - 0959-4493 (Print)
IS  - 0959-4493 (Linking)
VI  - 18
IP  - 4
DP  - 2007 Aug
TI  - Surveillance of healthy cats and cats with inflammatory skin disease for 
      colonization of the skin by methicillin-resistant coagulase-positive 
      staphylococci and Staphylococcus schleiferi ssp. schleiferi.
PG  - 252-9
AB  - In this study, bacterial cultures were collected from five sites on each of 50 
      healthy cats and 48 cats with inflammatory skin disease (ISD), to determine 
      prevalence of carriage and relative frequency of methicillin resistance in 
      coagulase-positive staphylococci and Staphylococcus schleiferi ssp. schleiferi. 
      Latex agglutination testing for penicillin-binding protein 2a (PBP2a) and pulsed 
      field gel electrophoresis (PFGE) were performed on all methicillin-resistant (MR) 
      isolates. Polymerase chain reaction (PCR) for the mecA gene was performed on MR 
      S. intermedius and S. schleiferi isolates. Staphylococcal chromosomal cassette 
      (SCCmec) typing was performed on all MR S. aureus isolates. Coagulase-positive 
      staphylococci and S. schleiferi ssp. schleiferi were isolated from 24 of 48 cats 
      with ISD: Staphylococcus aureus (14 of 24, 58%), Staphylococcus intermedius (11 
      of 24, 46%), Staphylococcus schleiferi ssp. schleiferi (1 of 24, 4%), and 
      Staphylococcus hyicus (1 of 24, 4%). Prevalence of MR was 7% for S. aureus, 0% 
      for S. intermedius, 100% for S. schleiferi ssp. schleiferi, and 0% for S. hyicus. 
      Coagulase-positive staphylococci were isolated from 17 of 50 healthy cats: S. 
      aureus (10 of 17, 59%), S. intermedius (11 of 17, 65%), and S. schleiferi ssp. 
      coagulans (1 of 17, 6%). Prevalence of MR was 20% for S. aureus, 18% for S. 
      intermedius, and 0% for S. schleiferi ssp. coagulans. All MR isolates were 
      positive for PBP2a via latex agglutination. Methicillin-resistant S. intermedius 
      and S. schleiferi ssp. schleiferi isolates were also positive for the mecA gene 
      via PCR. Methicillin-resistant S. aureus isolates were identified as SCCmec type 
      II. Results of PFGE indicated heterogeneity among isolates. There was no 
      significant difference in staphylococcal isolation or methicillin resistance 
      between study groups. While present, MR coagulase-positive staphylococci are 
      significantly less common in these study populations.
FAU - Abraham, Jill L
AU  - Abraham JL
AD  - Department of Clinical Studies, University of Pennsylvania School of Veterinary 
      Medicine, Philadelphia, PA, USA. abrahamj@vet.upenn.edu
FAU - Morris, Daniel O
AU  - Morris DO
FAU - Griffeth, Gregory C
AU  - Griffeth GC
FAU - Shofer, Frances S
AU  - Shofer FS
FAU - Rankin, Shelley C
AU  - Rankin SC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Vet Dermatol
JT  - Veterinary dermatology
JID - 9426187
RN  - 0 (DNA, Bacterial)
SB  - IM
MH  - Animals
MH  - Case-Control Studies
MH  - Cat Diseases/*epidemiology/etiology/*microbiology/pathology
MH  - Cats
MH  - DNA, Bacterial/analysis
MH  - Electrophoresis, Gel, Pulsed-Field/veterinary
MH  - Female
MH  - Male
MH  - Methicillin Resistance
MH  - Philadelphia/epidemiology
MH  - Polymerase Chain Reaction/veterinary
MH  - Population Surveillance
MH  - Staphylococcal Skin Infections/epidemiology/microbiology/*veterinary
MH  - Staphylococcus/classification/genetics/*isolation & purification
EDAT- 2007/07/06 09:00
MHDA- 2007/08/19 09:00
CRDT- 2007/07/06 09:00
PHST- 2007/07/06 09:00 [pubmed]
PHST- 2007/08/19 09:00 [medline]
PHST- 2007/07/06 09:00 [entrez]
AID - VDE604 [pii]
AID - 10.1111/j.1365-3164.2007.00604.x [doi]
PST - ppublish
SO  - Vet Dermatol. 2007 Aug;18(4):252-9. doi: 10.1111/j.1365-3164.2007.00604.x.