PMID- 17612591
OWN - NLM
STAT- MEDLINE
DCOM- 20070926
LR  - 20181113
IS  - 1673-7067 (Print)
IS  - 1995-8218 (Electronic)
IS  - 1995-8218 (Linking)
VI  - 23
IP  - 3
DP  - 2007 May
TI  - Therapeutic effect of microencapsulated porcine retinal pigmented epithelial 
      cells transplantation on rat model of Parkinson's disease.
PG  - 137-44
AB  - OBJECT: To investigate the therapeutic effect of microencapsulated porcine 
      retinal pigmented epithelial cells (RPE-M) transplantation on rat model of 
      Parkinson's disease (PD). METHODS: Primary porcine RPE cells were harvested by 
      enzyme digestion and expanded in culture medium. Determine the levels of dopamine 
      (DA) and homovanillic acid (HVA) by high performance liquid chromatography 
      electrochemical (HPLC) assay, and the levels of brain-derived neurotrophic factor 
      (BDNF) and glial-derived neurotrophic factor (GDNF) were detected by ELISA. 
      Alginate-polylysine-alginate (APA) microencapsulated cells were produced by using 
      a high voltage electrostatic system. PD rat model was established by unilateral 
      injection of 6-hydroxydopamine (6-OHDA) into the medial forebrain bundle (MFB). 
      After that, the RPE-M was transplanted into the corpus striatum of PD rat, and 
      then the rotation test scores were recorded and biochemical changes of the corpus 
      striatum were tested. RESULTS: The levels of DA, HVA, BDNF and GDNF secreted by 
      RPE were stable in the RPE culture supernatant and were not changed by the 
      microencapsulation. Eighty-three percent rats developed PD by unilateral lesion 
      of 6-OHDA in the MFB. The RPE-M transplantation had therapeutic effect on 33% PD 
      rats. CONCLUSION: Porcine RPE cells grow actively in vitro and could secrete DA, 
      HVA, BDNF, and GDNF constantly, which does not be affected by the passage culture 
      and the APA miroencapsulation. RPE-M transplantation of may be a curative therapy 
      for PD.
FAU - Zhang, Hou-Liang
AU  - Zhang HL
AD  - Department of Neurology, Huashan Hospital, Fudan University, Shanghai 200040, 
      China.
FAU - Wu, Jian-Jun
AU  - Wu JJ
FAU - Ren, Hui-Min
AU  - Ren HM
FAU - Wang, Jian
AU  - Wang J
FAU - Su, Ya-Ru
AU  - Su YR
FAU - Jiang, Yu-Ping
AU  - Jiang YP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Singapore
TA  - Neurosci Bull
JT  - Neuroscience bulletin
JID - 101256850
RN  - 0 (Adrenergic Agents)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Glial Cell Line-Derived Neurotrophic Factor)
RN  - 8HW4YBZ748 (Oxidopamine)
RN  - EC 1.14.16.2 (Tyrosine 3-Monooxygenase)
RN  - VTD58H1Z2X (Dopamine)
SB  - IM
MH  - Adrenergic Agents/toxicity
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Cell Transplantation/*methods
MH  - Cells, Cultured
MH  - Disease Models, Animal
MH  - Dopamine/metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Epithelial Cells/metabolism/*transplantation
MH  - Glial Cell Line-Derived Neurotrophic Factor/metabolism
MH  - Male
MH  - Oxidopamine/toxicity
MH  - Parkinson Disease/etiology/*surgery
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Retina/*cytology
MH  - Swine/anatomy & histology
MH  - Time Factors
MH  - Transplantation, Heterologous/methods
MH  - Tyrosine 3-Monooxygenase/metabolism
PMC - PMC5550627
EDAT- 2007/07/07 09:00
MHDA- 2007/09/27 09:00
PMCR- 2008/05/01
CRDT- 2007/07/07 09:00
PHST- 2007/07/07 09:00 [pubmed]
PHST- 2007/09/27 09:00 [medline]
PHST- 2007/07/07 09:00 [entrez]
PHST- 2008/05/01 00:00 [pmc-release]
AID - 20 [pii]
AID - 10.1007/s12264-007-0020-3 [doi]
PST - ppublish
SO  - Neurosci Bull. 2007 May;23(3):137-44. doi: 10.1007/s12264-007-0020-3.