PMID- 17612982
OWN - NLM
STAT- MEDLINE
DCOM- 20070822
LR  - 20091117
IS  - 1556-9527 (Print)
IS  - 1556-9527 (Linking)
VI  - 26
IP  - 2
DP  - 2007
TI  - The role of non-covalent protein binding in skin sensitisation potency of 
      chemicals.
PG  - 161-9
AB  - Skin sensitisation is a delayed hypersensitivity reaction caused by repeated 
      exposure to common natural and synthetic chemical allergens. It is thought that 
      small chemical sensitisers (haptens) are required to form a strong irreversible 
      bond with a self protein/peptide and generate an immunogenic hapten-protein 
      complex in order to be recognised by the immune system and stimulate T cell 
      proliferation. The sensitisers are usually electrophilic chemicals that are 
      directly reactive with proteins or reactive intermediates (metabolites) of 
      chemically inert compounds (prohaptens). Sensitising chemicals are also capable 
      of weak, non-covalent association with proteins and there is an ongoing debate 
      about the role of weak interactions of chemicals and proteins in the chemistry of 
      allergy. The non-covalent interactions are reversible and thus have a major 
      impact on skin/epidermal bioavailability of chemical/reactive metabolites. We 
      investigated the relationship between the relative level of non-covalent 
      association to a model protein and their relative potencies as determined by the 
      EC3 values in the murine local lymph node assay (LLNA) for a number of chemicals. 
      Using human serum albumin as a model protein, we determined that no observable 
      relationship exists between the two parameters for the chemicals tested. 
      Therefore, at least for this model protein, non-covalent interactions appear not 
      to be a key determinant of allergen potency.
FAU - Aleksic, Maja
AU  - Aleksic M
AD  - Safety & Environmental Assurance Centre Unilever Colworth, Sharnbrook, 
      Bedfordshire. maja.aleksic@unilever.com
FAU - Thain, Emma
AU  - Thain E
FAU - Gutsell, Stephen J
AU  - Gutsell SJ
FAU - Pease, Camilla K
AU  - Pease CK
FAU - Basketter, David A
AU  - Basketter DA
LA  - eng
PT  - Journal Article
PL  - England
TA  - Cutan Ocul Toxicol
JT  - Cutaneous and ocular toxicology
JID - 101266892
RN  - 0 (Allergens)
RN  - 0 (Haptens)
RN  - 0 (Serum Albumin)
SB  - IM
MH  - Allergens/*chemistry/*metabolism
MH  - Chromatography, Affinity
MH  - Chromatography, High Pressure Liquid
MH  - Dermatitis, Contact/*metabolism
MH  - Haptens/metabolism
MH  - Humans
MH  - Hypersensitivity, Delayed/*metabolism
MH  - Local Lymph Node Assay
MH  - Protein Binding
MH  - Serum Albumin/immunology
MH  - Spectrophotometry, Ultraviolet
EDAT- 2007/07/07 09:00
MHDA- 2007/08/23 09:00
CRDT- 2007/07/07 09:00
PHST- 2007/07/07 09:00 [pubmed]
PHST- 2007/08/23 09:00 [medline]
PHST- 2007/07/07 09:00 [entrez]
AID - 779876254 [pii]
AID - 10.1080/15569520701212282 [doi]
PST - ppublish
SO  - Cutan Ocul Toxicol. 2007;26(2):161-9. doi: 10.1080/15569520701212282.