PMID- 17613050
OWN - NLM
STAT- MEDLINE
DCOM- 20071119
LR  - 20131121
IS  - 0265-203X (Print)
IS  - 0265-203X (Linking)
VI  - 24
IP  - 7
DP  - 2007 Jul
TI  - Determination of acrylamide in coffee and chocolate by pressurised fluid 
      extraction and liquid chromatography-tandem mass spectrometry.
PG  - 663-72
AB  - A selective and sensitive procedure has been developed and validated for the 
      determination of acrylamide in difficult matrices, such as coffee and chocolate. 
      The proposed method includes pressurised fluid extraction (PFE) with 
      acetonitrile, florisil clean-up purification inside the PFE extraction cell and 
      detection by liquid chromatography (LC) coupled to atmospheric pressure 
      ionisation in positive mode tandem mass spectrometry (APCI-MS-MS). Comparison of 
      ionisation sources (atmospheric pressure chemical ionisation (APCI), atmospheric 
      pressure photoionization (APPI) and the combined APCI/APPI) and clean-up 
      procedures were carried out to improve the analytical signal. The main parameters 
      affecting the performance of the different ionisation sources were previously 
      optimised using statistical design of experiments (DOE). PFE parameters were also 
      optimised by DOE. For quantitation, an isotope dilution approach was used. The 
      limit of quantification (LOQ) of the method was 1 microg kg(-1) for coffee and 
      0.6 microg kg(-1) for chocolate. Recoveries ranged between 81-105% in coffee and 
      87-102% in chocolate. The accuracy was evaluated using a coffee reference test 
      material FAPAS T3008. Using the optimised method, 20 coffee and 15 chocolate 
      samples collected from Valencian (Spain) supermarkets, were investigated for 
      acrylamide, yielding median levels of 146 microg kg(-1) in coffee and 102 microg 
      kg(-1) in chocolate.
FAU - Pardo, O
AU  - Pardo O
AD  - Public Health Laboratory of Valencia, Generalitat Valenciana, Valencia, Spain.
FAU - Yusa, V
AU  - Yusa V
FAU - Coscolla, C
AU  - Coscolla C
FAU - Leon, N
AU  - Leon N
FAU - Pastor, A
AU  - Pastor A
LA  - eng
PT  - Journal Article
PL  - England
TA  - Food Addit Contam
JT  - Food additives and contaminants
JID - 8500474
RN  - 0 (Coffee)
RN  - 20R035KLCI (Acrylamide)
SB  - IM
MH  - Acrylamide/*analysis
MH  - Cacao/*chemistry
MH  - Chromatography, Liquid/methods
MH  - Coffee/*chemistry
MH  - Spain
MH  - Tandem Mass Spectrometry/methods
EDAT- 2007/07/07 09:00
MHDA- 2007/12/06 09:00
CRDT- 2007/07/07 09:00
PHST- 2007/07/07 09:00 [pubmed]
PHST- 2007/12/06 09:00 [medline]
PHST- 2007/07/07 09:00 [entrez]
AID - 779724068 [pii]
AID - 10.1080/02652030701235198 [doi]
PST - ppublish
SO  - Food Addit Contam. 2007 Jul;24(7):663-72. doi: 10.1080/02652030701235198.