PMID- 1761399
OWN - NLM
STAT- MEDLINE
DCOM- 19920207
LR  - 20190918
IS  - 0162-3109 (Print)
IS  - 0162-3109 (Linking)
VI  - 22
IP  - 2
DP  - 1991 Sep-Oct
TI  - Epithelial modulation of thromboxane A2 and PAF involvement in IgE- and 
      IgG-mediated guinea pig anaphylaxis.
PG  - 115-25
AB  - The role of prostanoids and platelet-activating factor (PAF) was studied in the 
      in vitro response of guinea pig trachea to immunochallenge according to the 
      presence or the absence of the epithelial layer and to the sensitization 
      procedure leading to the preferential synthesis of immunoglobulin E (IgE) or 
      immunoglobulin G (IgG) antibodies. Indomethacin, a cyclooxygenase inhibitor, 
      potentiated the antigen-induced contractions both in IgE and IgG models, 
      suggesting the involvement of relaxant prostaglandins (PGs), independently of the 
      presence of the airway epithelium. UK-38485, a thromboxane synthetase inhibitor, 
      did not modify the tracheal response to antigen in the IgE model. However, this 
      compound enhanced the maximum contractile response to antigen of the intact 
      tracheal strips of IgG-sensitized guinea pig, but reduced the contractile 
      response of the epithelium-free tracheal strips. Two potent non-structurally 
      related PAF antagonists, Ro 19-3704 and BN 52021, reduced antigen-induced 
      contraction of the epithelium-free tracheal strips in the IgE model. In contrast, 
      these compounds did not affect the contractile responses of the preparations in 
      the IgG model. These results suggest the selective implication of thromboxane A2 
      and PAF, in IgG- and IgE-mediated guinea pig anaphylaxis respectively. Finally, 
      these results indicate that thromboxane A2 (TXA2) and PAF are potent inducers of 
      epithelium-derived mediators.
FAU - Bertrand, C
AU  - Bertrand C
AD  - Laboratoire de Neuroimmunopharmacologie, Universite Louis Pasteur, Strasbourg I, 
      Faculte de Pharmacie, Illkirch, France.
FAU - Tschirhart, E
AU  - Tschirhart E
FAU - Landry, Y
AU  - Landry Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Immunopharmacology
JT  - Immunopharmacology
JID - 7902474
RN  - 0 (Antigens)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Platelet Activating Factor)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 57576-52-0 (Thromboxane A2)
RN  - 9006-59-1 (Ovalbumin)
RN  - XXE1CET956 (Indomethacin)
SB  - IM
MH  - Anaphylaxis/*etiology
MH  - Animals
MH  - Antigens
MH  - Disease Models, Animal
MH  - Epithelium/immunology
MH  - Guinea Pigs
MH  - Immunoglobulin E
MH  - Immunoglobulin G
MH  - In Vitro Techniques
MH  - Indomethacin/pharmacology
MH  - Male
MH  - Ovalbumin/immunology
MH  - Platelet Activating Factor/antagonists & inhibitors/*immunology
MH  - Thromboxane A2/antagonists & inhibitors/*immunology
MH  - Trachea/drug effects/immunology
EDAT- 1991/09/01 00:00
MHDA- 1991/09/01 00:01
CRDT- 1991/09/01 00:00
PHST- 1991/09/01 00:00 [pubmed]
PHST- 1991/09/01 00:01 [medline]
PHST- 1991/09/01 00:00 [entrez]
AID - 0162-3109(91)90036-X [pii]
AID - 10.1016/0162-3109(91)90036-x [doi]
PST - ppublish
SO  - Immunopharmacology. 1991 Sep-Oct;22(2):115-25. doi: 10.1016/0162-3109(91)90036-x.