PMID- 17615985
OWN - NLM
STAT- MEDLINE
DCOM- 20071030
LR  - 20070709
IS  - 1009-3591 (Print)
IS  - 1009-3591 (Linking)
VI  - 13
IP  - 6
DP  - 2007 Jun
TI  - [Chinese herbal medicine PC-SPES II induces the apoptosis of androgen-independent 
      prostate carcinoma cell line PC-3].
PG  - 563-7
AB  - OBJECTIVE: To study the action mechanism of a Chinese herbal mixture PC-SPES II 
      inducing the apoptosis of androgen independent prostate adenocarcinoma cell line 
      (PC-3). METHODS: The growth of PC-3 was shown by MTT. Immunofluorescence staining 
      of acridine orange (AO) and flow cytometry were used to detect the apoptosis. The 
      expressions of the apoptosis-related proteins were analyzed with their monoclonal 
      or polyclonal antibodies after Western blotting. RESULTS: PC-SPES II not only 
      inhibited the growth of PC-3 cells but also induced their death. The apoptosis of 
      PC-3 cells treated with PC-SPES II was detected by immunofluorescence staining of 
      AO and flow cytometry, which showed the apoptotic cells to be (29.8 +/- 5.6)%, 
      but the untreated control cells (0.06 +/- 0.014)%, (P < 0.01). The expression of 
      Bcl-2 and Bcl-xL, two antiapoptosis proteins, was decreased while Bax, a pro 
      apoptosis protein, was elevated in the cells treated with PC-SPES II as compared 
      with the untreated control (P < 0.01). Accordingly, the expression of the 
      activated fragments of caspase-3 was also increased (P < 0.01). CONCLUSION: The 
      Chinese herbal mixture PCSPES II can induce the apoptosis of PC-3. Its mechanism 
      may lie in the up-regulation of Bax expression and down-regulation of Bcl-2 and 
      Bcl-xL expressions, which induce the activated fragments of caspase-3 by 
      mitochondria.
FAU - Du, Guang
AU  - Du G
AD  - Department of Urology, ST. Luke's Hospital, Shanghai 200050, China.
FAU - Zhang, Zheng-wang
AU  - Zhang ZW
FAU - Zhang, Yong-kang
AU  - Zhang YK
FAU - Guo, Jian-ming
AU  - Guo JM
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Nan Ke Xue
JT  - Zhonghua nan ke xue = National journal of andrology
JID - 101093592
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (herbal preparation PC-SPES)
SB  - IM
MH  - Apoptosis/*drug effects
MH  - Cell Line, Tumor
MH  - Drugs, Chinese Herbal/*pharmacology/therapeutic use
MH  - Humans
MH  - Male
MH  - *Phytotherapy
MH  - Prostatic Neoplasms/*drug therapy/pathology
EDAT- 2007/07/10 09:00
MHDA- 2007/10/31 09:00
CRDT- 2007/07/10 09:00
PHST- 2007/07/10 09:00 [pubmed]
PHST- 2007/10/31 09:00 [medline]
PHST- 2007/07/10 09:00 [entrez]
PST - ppublish
SO  - Zhonghua Nan Ke Xue. 2007 Jun;13(6):563-7.