PMID- 17617439
OWN - NLM
STAT- MEDLINE
DCOM- 20080111
LR  - 20161124
IS  - 0045-6535 (Print)
IS  - 0045-6535 (Linking)
VI  - 69
IP  - 10
DP  - 2007 Nov
TI  - Mineralization of PAHs in coal-tar impacted aquifer sediments and associated 
      microbial community structure investigated with FISH.
PG  - 1563-73
AB  - The microbial community structure and mineralization of polycyclic aromatic 
      hydrocarbons (PAHs) in a coal-tar contaminated aquifer were investigated 
      spatially using fluorescence in situ hybridization (FISH) and in laboratory-scale 
      incubations of the aquifer sediments. DAPI-detected microbial populations in the 
      contaminated sediments were three orders of magnitude greater than nearby 
      uncontaminated sediments, suggesting growth on coal-tar constituents in situ. 
      Actinobacteria, beta- and gamma-Proteobacteria, and Flavobacteria dominated the 
      in situ aerobic (>1 mg l(-1) dissolved oxygen) microbial community, whereas 
      sulfate-reducing bacteria comprised 37% of the microbial community in the 
      sulfidogenic region of the aquifer. Rapid mineralization of naphthalene and 
      phenanthrene were observed in aerobic laboratory microcosms and resulted in 
      significant enrichment of beta- and gamma-Proteobacteria potentially explaining 
      their elevated presence in situ. Firmicutes, Flavobacteria, alpha-Proteobacteria, 
      and Actinobacteria were also enriched in the mineralization assays, but to a 
      lesser degree. Nitrate- and sulfate-limited mineralization of naphthalene in 
      laboratory microcosms occurred to a small degree in aquifer sediments from 
      locations where groundwater chemistry indicated nitrate- and sulfate-reduction, 
      respectively. Some iron-limited mineralization of naphthalene and phenanthrene 
      was also observed in sediments originating near groundwater measurements of 
      elevated ferrous iron. The results of this study suggest that FISH may be a 
      useful tool for providing a much needed link between laboratory microcosms and 
      groundwater measurements made in situ necessary to better demonstrate the 
      potential for natural attenuation at complex PAH contaminated sites.
FAU - Rogers, Shane W
AU  - Rogers SW
AD  - Department of Civil, Construction and Environmental Engineering, Iowa State 
      University, Ames, IA 50011, USA. swrogers@cafoexpert.com
FAU - Ong, Say Kee
AU  - Ong SK
FAU - Moorman, Thomas B
AU  - Moorman TB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070706
PL  - England
TA  - Chemosphere
JT  - Chemosphere
JID - 0320657
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (Polycyclic Aromatic Hydrocarbons)
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (RNA, Ribosomal, 23S)
RN  - 0 (Water Pollutants, Chemical)
RN  - 8007-45-2 (Coal Tar)
SB  - IM
MH  - Bacteria, Aerobic/growth & development/isolation & purification
MH  - Bacteria, Anaerobic/growth & development/isolation & purification
MH  - Biodegradation, Environmental
MH  - Coal Tar/*analysis/chemistry
MH  - Geologic Sediments/*chemistry/*microbiology
MH  - In Situ Hybridization, Fluorescence
MH  - Oligonucleotide Probes
MH  - Polycyclic Aromatic Hydrocarbons/*analysis/chemistry
MH  - RNA, Bacterial/analysis
MH  - RNA, Ribosomal, 16S/analysis
MH  - RNA, Ribosomal, 23S/analysis
MH  - Water Pollutants, Chemical/*analysis/chemistry
EDAT- 2007/07/10 09:00
MHDA- 2008/01/12 09:00
CRDT- 2007/07/10 09:00
PHST- 2006/12/06 00:00 [received]
PHST- 2007/05/23 00:00 [revised]
PHST- 2007/05/24 00:00 [accepted]
PHST- 2007/07/10 09:00 [pubmed]
PHST- 2008/01/12 09:00 [medline]
PHST- 2007/07/10 09:00 [entrez]
AID - S0045-6535(07)00716-3 [pii]
AID - 10.1016/j.chemosphere.2007.05.058 [doi]
PST - ppublish
SO  - Chemosphere. 2007 Nov;69(10):1563-73. doi: 10.1016/j.chemosphere.2007.05.058. 
      Epub 2007 Jul 6.