PMID- 17617571
OWN - NLM
STAT- MEDLINE
DCOM- 20070919
LR  - 20190516
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 179
IP  - 2
DP  - 2007 Jul 15
TI  - Dendritic cells trigger tumor cell death by a nitric oxide-dependent mechanism.
PG  - 812-8
AB  - Dendritic cells (DCs) are well known for their capacity to induce adaptive 
      antitumor immune response through Ag presentation and tumor-specific T cell 
      activation. Recent findings reveal that besides this role, DCs may display 
      additional antitumor effects. In this study, we provide evidence that LPS- or 
      IFN-gamma-activated rat bone marrow-derived dendritic cells (BMDCs) display 
      killing properties against tumor cells. These cytotoxic BMDCs exhibit a mature DC 
      phenotype, produce high amounts of IL-12, IL-6, and TNF-alpha, and retain their 
      phagocytic properties. BMDC-mediated tumor cell killing requires cell-cell 
      contact and depends on NO production, but not on perforin/granzyme or on death 
      receptors. Furthermore, dead tumor cells do not exhibit characteristics of 
      apoptosis. Thus, intratumoral LPS injections induce an increase of inducible NO 
      synthase expression in tumor-infiltrating DCs associated with a significant 
      arrest of tumor growth. Altogether, these results suggest that LPS-activated 
      BMDCs represent powerful tumoricidal cells which enforce their potential as 
      anticancer cellular vaccines.
FAU - Nicolas, Alexandra
AU  - Nicolas A
AD  - Institut National de la Sante et de la Recherche Medicale Unit Mixte de Recherche 
      866, Institut Federatif de Recherche 100, Universite de Bourgogne, Dijon, France.
FAU - Cathelin, Dominique
AU  - Cathelin D
FAU - Larmonier, Nicolas
AU  - Larmonier N
FAU - Fraszczak, Jennifer
AU  - Fraszczak J
FAU - Puig, Pierre-Emmanuel
AU  - Puig PE
FAU - Bouchot, Andre
AU  - Bouchot A
FAU - Bateman, Andrew
AU  - Bateman A
FAU - Solary, Eric
AU  - Solary E
FAU - Bonnotte, Bernard
AU  - Bonnotte B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Lipopolysaccharides)
RN  - 31C4KY9ESH (Nitric Oxide)
SB  - IM
MH  - Adjuvants, Immunologic/pharmacology
MH  - Animals
MH  - Blotting, Western
MH  - Bone Marrow Cells/immunology
MH  - Cell Death/*physiology
MH  - Cell Line, Tumor
MH  - *Cytotoxicity, Immunologic
MH  - Dendritic Cells/drug effects/*metabolism
MH  - Flow Cytometry
MH  - Humans
MH  - Lipopolysaccharides/pharmacology
MH  - Neoplasms/*immunology
MH  - Nitric Oxide/*metabolism
MH  - Rats
EDAT- 2007/07/10 09:00
MHDA- 2007/09/20 09:00
CRDT- 2007/07/10 09:00
PHST- 2007/07/10 09:00 [pubmed]
PHST- 2007/09/20 09:00 [medline]
PHST- 2007/07/10 09:00 [entrez]
AID - 179/2/812 [pii]
AID - 10.4049/jimmunol.179.2.812 [doi]
PST - ppublish
SO  - J Immunol. 2007 Jul 15;179(2):812-8. doi: 10.4049/jimmunol.179.2.812.