PMID- 17617837
OWN - NLM
STAT- MEDLINE
DCOM- 20070927
LR  - 20220316
IS  - 0904-2512 (Print)
IS  - 0904-2512 (Linking)
VI  - 36
IP  - 7
DP  - 2007 Aug
TI  - Requirement of MMP-3 in anchorage-independent growth of oral squamous cell 
      carcinomas.
PG  - 430-5
AB  - BACKGROUND: Matrix metalloproteinase-3 (MMP-3) is expressed in various 
      carcinomas; however, its function is not clearly established. This study was to 
      assess its possible role in oral squamous cell carcinomas (OSCCs). MATERIALS AND 
      METHODS: Specimens of seven oral pre-malignant lesions (OPMLs) and 92 OSCCs were 
      subjected to MMP-3 detection by RT-PCR and Western blot. Antisense 
      oligodeoxynucleotides (AODNs) of MMP-3 were used to transfect OSCC (OECM-1 and 
      SCC-9) and esophageal carcinoma (CE81T/VGH) cell lines, and their growth was 
      subsequently analyzed by XTT and soft-agar colony assay. RESULTS: MMP-3 
      transcript was preferentially expressed in OSCCs (71 of 92, 77%) than in OPMLs 
      (two of seven, 29%; P = 0.012). Both MMP-3 transcript and protein levels were 
      significantly higher in OSCC masses than in neighboring tissues (P < 0.0001 and P 
      = 0.04, respectively). Growth of the three cell lines was not affected, while the 
      colony numbers of OECM-1 and CE81T/VGH were significantly reduced by the 
      transfection of MMP-3 AODNs (P = 0.002 and P = 0.004, respectively). SCC-9 did 
      not form colonies in soft-agar/medium. CONCLUSIONS: MMP-3 function may be 
      required in most OSCCs, and it may support the anchorage-independent growth of 
      both OSCC and esophageal carcinoma.
FAU - Liu, Shyun-Yeu
AU  - Liu SY
AD  - Oral and Maxillofacial Surgery Section, Chi Mei Medical Center, Tainan, Taiwan.
FAU - Liu, Young-Chau
AU  - Liu YC
FAU - Huang, Wen-Tsung
AU  - Huang WT
FAU - Huang, Guan-Cheng
AU  - Huang GC
FAU - Su, Hsiao-Jing
AU  - Su HJ
FAU - Lin, Mei-Huei
AU  - Lin MH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - J Oral Pathol Med
JT  - Journal of oral pathology & medicine : official publication of the International 
      Association of Oral Pathologists and the American Academy of Oral Pathology
JID - 8911934
RN  - 0 (DNA, Antisense)
RN  - 0 (Indicators and Reagents)
RN  - 0 (Tetrazolium Salts)
RN  - 117038-70-7 
      (2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-((phenylamino)carbonyl)-2H-tetrazolium 
      hydroxide)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Carcinoma/genetics/pathology
MH  - Carcinoma, Squamous Cell/*enzymology
MH  - Cell Count
MH  - Cell Division/genetics
MH  - Cell Line, Tumor
MH  - DNA, Antisense/genetics
MH  - Esophageal Neoplasms/genetics/pathology
MH  - Gene Expression Regulation, Neoplastic/genetics
MH  - Humans
MH  - Indicators and Reagents
MH  - Matrix Metalloproteinase 3/genetics/*physiology
MH  - Mouth Neoplasms/*enzymology
MH  - Precancerous Conditions/enzymology
MH  - Tetrazolium Salts
MH  - Transfection
EDAT- 2007/07/10 09:00
MHDA- 2007/09/28 09:00
CRDT- 2007/07/10 09:00
PHST- 2007/07/10 09:00 [pubmed]
PHST- 2007/09/28 09:00 [medline]
PHST- 2007/07/10 09:00 [entrez]
AID - JOP524 [pii]
AID - 10.1111/j.1600-0714.2007.00524.x [doi]
PST - ppublish
SO  - J Oral Pathol Med. 2007 Aug;36(7):430-5. doi: 10.1111/j.1600-0714.2007.00524.x.