PMID- 17618110
OWN - NLM
STAT- MEDLINE
DCOM- 20080226
LR  - 20090714
IS  - 0956-5663 (Print)
IS  - 0956-5663 (Linking)
VI  - 23
IP  - 4
DP  - 2007 Nov 30
TI  - Microfluidic systems integrated with two-dimensional surface plasmon resonance 
      phase imaging systems for microarray immunoassay.
PG  - 466-72
AB  - This study reports a microfluidic chip integrated with an arrayed immunoassay for 
      surface plasmon resonance (SPR) phase imaging of specific bio-samples. The SPR 
      phase imaging system uses a surface-sensitive optical technique to detect 
      two-dimensional (2D) spatial phase variation caused by rabbit immunoglobulin G 
      (IgG) adsorbed on an anti-rabbit IgG film. The microfluidic chip was fabricated 
      by using micro-electro-mechanical-systems (MEMS) technology on glass and 
      polydimethylsiloxane (PDMS) substrates to facilitate well-controlled and 
      reproducible sample delivery and detection. Since SPR detection is very sensitive 
      to temperature variation, a micromachine-based temperature control module 
      comprising micro-heaters and temperature sensors was used to maintain a uniform 
      temperature distribution inside the arrayed detection area with a variation of 
      less than 0.3 degrees C. A self-assembled monolayer (SAM) technique was used to 
      pattern the surface chemistry on a gold layer to immobilize anti-rabbit IgG on 
      the modified substrates. The microfluidic chip is capable of transporting a 
      precise amount of IgG solution by using micropumps/valves to the arrayed 
      detection area such that highly sensitive, highly specific bio-sensing can be 
      achieved. The developed microfluidic chips, which employed SPR phase imaging for 
      immunoassay analysis, could successfully detect the interaction of anti-rabbit 
      IgG and IgG. The interactions between immobilized anti-rabbit IgG and IgG with 
      various concentrations have been measured. The detection limit is experimentally 
      found to be 1 x 10(-4)mg/ml (0.67 nM). The specificity of the arrayed immunoassay 
      was also explored. Experimental data show that only the rabbit IgG can be 
      detected and the porcine IgG cannot be adsorbed. The developed microfluidic 
      system is promising for various applications including medical diagnostics, 
      microarray detection and observing protein-protein interactions.
FAU - Lee, Kuo-Hoong
AU  - Lee KH
AD  - Department of Engineering Science, National Cheng Kung University, Taiwan.
FAU - Su, Yuan-Deng
AU  - Su YD
FAU - Chen, Shean-Jen
AU  - Chen SJ
FAU - Tseng, Fan-Gang
AU  - Tseng FG
FAU - Lee, Gwo-Bin
AU  - Lee GB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070602
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
SB  - IM
MH  - Biosensing Techniques/instrumentation/*methods
MH  - Immunoassay/instrumentation/methods
MH  - Microfluidic Analytical Techniques/instrumentation/*methods
MH  - Reproducibility of Results
MH  - Surface Plasmon Resonance/instrumentation/*methods
MH  - Temperature
EDAT- 2007/07/10 09:00
MHDA- 2008/02/27 09:00
CRDT- 2007/07/10 09:00
PHST- 2007/02/24 00:00 [received]
PHST- 2007/05/10 00:00 [revised]
PHST- 2007/05/22 00:00 [accepted]
PHST- 2007/07/10 09:00 [pubmed]
PHST- 2008/02/27 09:00 [medline]
PHST- 2007/07/10 09:00 [entrez]
AID - S0956-5663(07)00263-1 [pii]
AID - 10.1016/j.bios.2007.05.007 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2007 Nov 30;23(4):466-72. doi: 10.1016/j.bios.2007.05.007. 
      Epub 2007 Jun 2.