PMID- 17618126
OWN - NLM
STAT- MEDLINE
DCOM- 20071107
LR  - 20181113
IS  - 0969-9961 (Print)
IS  - 1095-953X (Electronic)
IS  - 0969-9961 (Linking)
VI  - 27
IP  - 2
DP  - 2007 Aug
TI  - Strategies for promoting anti-seizure effects of hippocampal fetal cells grafted 
      into the hippocampus of rats exhibiting chronic temporal lobe epilepsy.
PG  - 117-32
AB  - Efficacy of hippocampal fetal cell (HFC) grafting for restraining spontaneous 
      recurrent motor seizures (SRMS) in chronic temporal lobe epilepsy (TLE) is 
      unknown. We investigated both survival and anti-seizure effects of 
      5'-bromodeoxyuridine (BrdU) labeled embryonic day 19 (E19) HFC grafts pretreated 
      with different neurotrophic factors and a caspase inhibitor. Grafts were placed 
      bilaterally into the hippocampi of F344 rats exhibiting kainate (KA) induced 
      chronic TLE, where the frequency of SRMS varied from 3.0 to 3.5 seizures/8-h 
      duration. The first group received standard (untreated) HFC grafts, the second 
      group received HFC grafts pretreated and transplanted with brain-derived 
      neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and caspase inhibitor 
      Ac-YVAD-cmk (BNC-treated HFC grafts), the third group received HFC grafts 
      pretreated and transplanted with fibroblast growth factor-2 (FGF-2) and caspase 
      inhibitor Ac-YVAD-cmk (FC-treated HFC grafts), and the fourth group served as 
      epilepsy-only controls. Epileptic rats receiving standard HFC grafts exhibited 
      119% increase in the frequency of SRMS at 2 months post-grafting consistent with 
      125% increase in seizure frequency observed in epilepsy-only controls during the 
      same period. However, in epileptic rats receiving HFC grafts treated with BNC or 
      FC, the frequency of SRMS was 33-39% less than their pre-transplant scores and 
      73-76% less than rats receiving standard HFC grafts or epilepsy-only rats. The 
      yield of surviving neurons was equivalent to 30% of injected cells in standard 
      HFC grafts, 57% in HFC grafts treated with BNC and 98% in HFC grafts treated with 
      FC. Thus, standard HFC grafts survive poorly in the chronically epileptic 
      hippocampus and fail to restrain the progression of chronic TLE. In contrast, 
      HFCs treated and grafted with BNC or FC survive robustly in the chronically 
      epileptic hippocampus, considerably reduce the frequency of SRMS and blunt the 
      progression of chronic TLE.
FAU - Rao, Muddanna S
AU  - Rao MS
AD  - Department of Surgery (Neurosurgery) Duke University Medical Center, Durham, NC 
      27710, USA.
FAU - Hattiangady, Bharathi
AU  - Hattiangady B
FAU - Rai, Kiranmai S
AU  - Rai KS
FAU - Shetty, Ashok K
AU  - Shetty AK
LA  - eng
GR  - R01 NS043507/NS/NINDS NIH HHS/United States
GR  - R01 NS054780/NS/NINDS NIH HHS/United States
GR  - R01 NS 043507/NS/NINDS NIH HHS/United States
GR  - R01 NS 54780/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20070523
PL  - United States
TA  - Neurobiol Dis
JT  - Neurobiology of disease
JID - 9500169
RN  - 0 (Amino Acid Chloromethyl Ketones)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Cysteine Proteinase Inhibitors)
RN  - 0 (N-acetyl-tyrosyl-valyl-alanyl-aspartyl chloromethyl ketone)
RN  - 0 (Neurotrophin 3)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
SB  - IM
MH  - Amino Acid Chloromethyl Ketones/metabolism
MH  - Animals
MH  - Brain Tissue Transplantation/*methods
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Cell Differentiation/physiology
MH  - Cysteine Proteinase Inhibitors/metabolism
MH  - Epilepsy, Temporal Lobe/complications/*surgery
MH  - Fetal Tissue Transplantation/*methods
MH  - Fibroblast Growth Factor 2/metabolism
MH  - Fluorescent Antibody Technique
MH  - Graft Survival/physiology
MH  - Hippocampus/*transplantation
MH  - Male
MH  - Neurons/cytology/metabolism/transplantation
MH  - Neurotrophin 3/metabolism
MH  - Rats
MH  - Rats, Inbred F344
MH  - Seizures/etiology/surgery
PMC - PMC3612502
MID - NIHMS439494
EDAT- 2007/07/10 09:00
MHDA- 2007/11/08 09:00
PMCR- 2013/03/31
CRDT- 2007/07/10 09:00
PHST- 2006/12/08 00:00 [received]
PHST- 2007/03/22 00:00 [revised]
PHST- 2007/03/27 00:00 [accepted]
PHST- 2007/07/10 09:00 [pubmed]
PHST- 2007/11/08 09:00 [medline]
PHST- 2007/07/10 09:00 [entrez]
PHST- 2013/03/31 00:00 [pmc-release]
AID - S0969-9961(07)00067-8 [pii]
AID - 10.1016/j.nbd.2007.03.016 [doi]
PST - ppublish
SO  - Neurobiol Dis. 2007 Aug;27(2):117-32. doi: 10.1016/j.nbd.2007.03.016. Epub 2007 
      May 23.