PMID- 17619252
OWN - NLM
STAT- MEDLINE
DCOM- 20070821
LR  - 20191210
IS  - 1121-7138 (Print)
IS  - 1121-7138 (Linking)
VI  - 30
IP  - 2
DP  - 2007 Apr
TI  - Envelope glycoproteins of hantavirus can mediate cell-cell fusion independently.
PG  - 101-7
AB  - Hantaviruses (HTVs) are enveloped viruses and can induce low PH-dependent cell 
      fusion. In this report we molecularly cloned viral glycoproteins (GPs) cDNA and 
      nucleocapsid (NP) cDNA of two strains of Hantaan virus and one strain of Seoul 
      virus and expressed in Vero E6 cells under control of a CMV promoter. The 
      examinations of viral gene expressions were carried out by IFA and 
      immune-precipitation. After treatment with low PH (PH 5.8) medium the syncytium 
      were observed in the cells transfected with the GPs clones while in the cells 
      transfected with the NP clones we did not find this phenomenon. Furthermore 
      cotransfection of the NP and GPs did not enhance fusion activity. Treatment with 
      anti-GP monoclonal antibodies could inhibit fusion activity whereas the 
      antibodies against NP could not. These results indicated that GPs can mediate 
      cell-cell fusion independently.
FAU - Zheng, Feng
AU  - Zheng F
AD  - Qilu Hospital of Shandong University, Jinan, Shandong Province, China.
FAU - Ma, Lixian
AU  - Ma L
FAU - Shao, Lihua
AU  - Shao L
FAU - Wang, Gang
AU  - Wang G
FAU - Chen, Fengzhe
AU  - Chen F
FAU - Zhang, Ying
AU  - Zhang Y
FAU - Yang, Song
AU  - Yang S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Italy
TA  - New Microbiol
JT  - The new microbiologica
JID - 9516291
RN  - 0 (Nucleocapsid Proteins)
RN  - 0 (Viral Envelope Proteins)
SB  - IM
MH  - Animals
MH  - *Cell Fusion
MH  - Chlorocebus aethiops
MH  - Cloning, Molecular
MH  - Fluorescent Antibody Technique, Indirect
MH  - Gene Expression
MH  - Giant Cells/cytology
MH  - Hantaan virus/*physiology
MH  - Hydrogen-Ion Concentration
MH  - Immunoprecipitation
MH  - Nucleocapsid Proteins/genetics/*physiology
MH  - Seoul virus/*physiology
MH  - Vero Cells
MH  - Viral Envelope Proteins/genetics/*physiology
EDAT- 2007/07/11 09:00
MHDA- 2007/08/22 09:00
CRDT- 2007/07/11 09:00
PHST- 2007/07/11 09:00 [pubmed]
PHST- 2007/08/22 09:00 [medline]
PHST- 2007/07/11 09:00 [entrez]
PST - ppublish
SO  - New Microbiol. 2007 Apr;30(2):101-7.