PMID- 17624433
OWN - NLM
STAT- MEDLINE
DCOM- 20071109
LR  - 20070928
IS  - 0161-5890 (Print)
IS  - 0161-5890 (Linking)
VI  - 45
IP  - 1
DP  - 2008 Jan
TI  - Molecular cloning and expression analysis of T-bet in ginbuna crucian carp 
      (Carassius auratus langsdorfii).
PG  - 127-36
AB  - In the adaptive immune system of mammals, naive helper T (Th) cells differentiate 
      into Th1 or Th2 cells. The T-box expressed in T cells (T-bet) is a member of a 
      family of T-box transcription factors that regulates the expression of IFN-gamma 
      and plays a crucial role in Th1 cell differentiation and cell-mediated immunity. 
      We cloned and sequenced T-bet cDNA for the first time from non-mammalian species, 
      ginbuna crucian carp. Ginbuna T-bet was composed of 608 predicted amino acids and 
      showed 41.5% identity with human T-bet (Tbx21), and human and ginbuna T-bet share 
      77.3% identity in their T-box regions. Comparative genomic analysis showed 
      conserved synteny in these regions between zebrafish, fugu, medaka and human 
      T-bet. Phylogenetic analysis indicated that ginbuna T-bet is closely related to 
      that of mouse and human. In unstimulated fish, ginbuna T-bet mRNA was strongly 
      expressed in peripheral blood leukocytes (PBL), head kidney (HK) and spleen. 
      RT-PCR analysis in kidney cells sorted by FACS revealed that T-bet was strongly 
      expressed in surface-IgM-negative lymphocytes in comparison to IgM-positive 
      lymphocytes. These results suggest that ginbuna T-bet is involved in the immune 
      system, especially in T-cell function, and is an important tool to analyze 
      teleost cell-mediated immunity.
FAU - Takizawa, Fumio
AU  - Takizawa F
AD  - Department of Veterinary Medicine, Nihon University, Fujisawa, Kanagawa 252-8510, 
      Japan.
FAU - Araki, Kyosuke
AU  - Araki K
FAU - Kobayashi, Isao
AU  - Kobayashi I
FAU - Moritomo, Tadaaki
AU  - Moritomo T
FAU - Ototake, Mitsuru
AU  - Ototake M
FAU - Nakanishi, Teruyuki
AU  - Nakanishi T
LA  - eng
SI  - GENBANK/AB290187
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070710
PL  - England
TA  - Mol Immunol
JT  - Molecular immunology
JID - 7905289
RN  - 0 (RNA, Messenger)
RN  - 0 (T-Box Domain Proteins)
RN  - 0 (T-box transcription factor TBX21)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Chromosomes/genetics
MH  - Cloning, Molecular
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation
MH  - Goldfish/*genetics
MH  - Humans
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - RNA, Messenger/genetics/metabolism
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
MH  - Synteny/genetics
MH  - T-Box Domain Proteins/chemistry/*genetics/metabolism
MH  - Tissue Distribution
EDAT- 2007/07/13 09:00
MHDA- 2007/11/10 09:00
CRDT- 2007/07/13 09:00
PHST- 2007/03/29 00:00 [received]
PHST- 2007/05/06 00:00 [accepted]
PHST- 2007/07/13 09:00 [pubmed]
PHST- 2007/11/10 09:00 [medline]
PHST- 2007/07/13 09:00 [entrez]
AID - S0161-5890(07)00218-0 [pii]
AID - 10.1016/j.molimm.2007.05.005 [doi]
PST - ppublish
SO  - Mol Immunol. 2008 Jan;45(1):127-36. doi: 10.1016/j.molimm.2007.05.005. Epub 2007 
      Jul 10.