PMID- 17626201
OWN - NLM
STAT- MEDLINE
DCOM- 20070807
LR  - 20200225
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Print)
IS  - 0270-6474 (Linking)
VI  - 27
IP  - 28
DP  - 2007 Jul 11
TI  - Mode of action and functional significance of estrogen-inducing dendritic growth, 
      spinogenesis, and synaptogenesis in the developing Purkinje cell.
PG  - 7408-17
AB  - Neurosteroids are synthesized de novo from cholesterol in the brain. To 
      understand neurosteroid action in the brain, data on the regio- and 
      temporal-specific synthesis of neurosteroids are needed. Recently, we identified 
      the Purkinje cell as an active neurosteroidogenic cell. In rodents, this neuron 
      actively produces several neurosteroids including estradiol during neonatal life, 
      when cerebellar neuronal circuit formation occurs. Estradiol may be involved in 
      cerebellar neuronal circuit formation through promoting neuronal growth and 
      neuronal synaptic contact, because the Purkinje cell expresses estrogen 
      receptor-beta (ERbeta). To test this hypothesis, in this study we examined the 
      effects of estradiol on dendritic growth, spinogenesis, and synaptogenesis in the 
      Purkinje cell using neonatal wild-type (WT) mice or cytochrome P450 aromatase 
      knock-out (ArKO) mice. Administration of estradiol to neonatal WT or ArKO mice 
      increased dendritic growth, spinogenesis, and synaptogenesis in the Purkinje 
      cell. In contrast, WT mice treated with tamoxifen, an ER antagonist, or ArKO mice 
      exhibited decreased Purkinje dendritic growth, spinogenesis, and synaptogenesis 
      at the same neonatal period. To elucidate the mode of action of estradiol, we 
      further examined the expression of brain-derived neurotrophic factor (BDNF) in 
      response to estrogen actions in the neonate. Estrogen administration to neonatal 
      WT or ArKO mice increased the BDNF level in the cerebellum, whereas tamoxifen 
      decreased the BDNF level in WT mice similar to ArKO mice. BDNF administration to 
      tamoxifen-treated WT mice increased Purkinje dendritic growth. These results 
      indicate that estradiol induces dendritic growth, spinogenesis, and 
      synaptogenesis in the developing Purkinje cell via BDNF action during neonatal 
      life.
FAU - Sasahara, Katsunori
AU  - Sasahara K
AD  - Laboratory of Brain Science, Faculty of Integrated Arts and Sciences, Hiroshima 
      University, Higashi-Hiroshima 739-8521, Japan.
FAU - Shikimi, Hanako
AU  - Shikimi H
FAU - Haraguchi, Shogo
AU  - Haraguchi S
FAU - Sakamoto, Hirotaka
AU  - Sakamoto H
FAU - Honda, Shin-ichiro
AU  - Honda S
FAU - Harada, Nobuhiro
AU  - Harada N
FAU - Tsutsui, Kazuyoshi
AU  - Tsutsui K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Estrogen Antagonists)
RN  - 0 (Estrogens)
RN  - 094ZI81Y45 (Tamoxifen)
RN  - 4TI98Z838E (Estradiol)
RN  - EC 1.14.14.1 (Aromatase)
SB  - IM
MH  - Aging/physiology
MH  - Animals
MH  - Animals, Newborn
MH  - Aromatase/deficiency
MH  - Brain-Derived Neurotrophic Factor/antagonists & inhibitors/metabolism/physiology
MH  - Cerebellum/drug effects/metabolism
MH  - Dendrites/drug effects/*physiology
MH  - Dendritic Spines/drug effects/*physiology
MH  - Estradiol/pharmacology
MH  - Estrogen Antagonists/pharmacology
MH  - Estrogens/*biosynthesis
MH  - Female
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Purkinje Cells/metabolism/*physiology
MH  - Synapses/drug effects/*physiology
MH  - Tamoxifen/pharmacology
PMC - PMC6672615
EDAT- 2007/07/13 09:00
MHDA- 2007/08/08 09:00
PMCR- 2008/01/11
CRDT- 2007/07/13 09:00
PHST- 2007/07/13 09:00 [pubmed]
PHST- 2007/08/08 09:00 [medline]
PHST- 2007/07/13 09:00 [entrez]
PHST- 2008/01/11 00:00 [pmc-release]
AID - 27/28/7408 [pii]
AID - 3243346 [pii]
AID - 10.1523/JNEUROSCI.0710-07.2007 [doi]
PST - ppublish
SO  - J Neurosci. 2007 Jul 11;27(28):7408-17. doi: 10.1523/JNEUROSCI.0710-07.2007.