PMID- 17626221
OWN - NLM
STAT- MEDLINE
DCOM- 20070807
LR  - 20200225
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Print)
IS  - 0270-6474 (Linking)
VI  - 27
IP  - 28
DP  - 2007 Jul 11
TI  - Anterograde transport and secretion of brain-derived neurotrophic factor along 
      sensory axons promote Schwann cell myelination.
PG  - 7597-603
AB  - The neurotrophin brain-derived neurotrophic factor (BDNF) inhibits Schwann cell 
      (SC) migration and promotes myelination via the p75 neurotrophin receptor (NTR). 
      Despite these recent findings, the expression, localization, and mechanism of 
      BDNF action has yet to be determined. Here we demonstrate that the sensory 
      neurons of the dorsal root ganglion (DRG) are a major source of BDNF during 
      postnatal development. The expression of BDNF is initially elevated before 
      myelination and decreases dramatically after the onset of myelination. BDNF 
      expression is controlled in part by transcriptional regulation and the increased 
      expression of the truncated TrkB receptor on SCs. To investigate the possible 
      mechanism of BDNF transport and release, multicompartment Campenot chambers were 
      used. DRG neurons transported and secreted endogenous BDNF along the surface of 
      axons in anterograde fashion. In an attempt to enhance myelination by SCs, DRG 
      neurons were transduced with an adenovirus to overexpress BDNF. BDNF was 
      transported and secreted along the axons and enhanced myelination when compared 
      with control cocultures. Together, the events surrounding the expression, 
      localization, and mechanism of BDNF action in DRG neurons may hint at potential 
      therapeutic implications to efficiently promote remyelination.
FAU - Ng, Benjamin K
AU  - Ng BK
AD  - Zilkha Neurogenetic Institute, Keck School of Medicine, University of Southern 
      California, Los Angeles, California 90033, USA.
FAU - Chen, Lian
AU  - Chen L
FAU - Mandemakers, Wilhelm
AU  - Mandemakers W
FAU - Cosgaya, Jose M
AU  - Cosgaya JM
FAU - Chan, Jonah R
AU  - Chan JR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Brain-Derived Neurotrophic Factor)
SB  - IM
MH  - Adenoviridae/genetics
MH  - Animals
MH  - Axons/*metabolism
MH  - Biological Transport
MH  - Brain-Derived Neurotrophic Factor/genetics/*metabolism
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Ganglia, Spinal/cytology/metabolism
MH  - Gene Transfer Techniques
MH  - Genetic Vectors
MH  - Humans
MH  - Myelin Sheath/*physiology
MH  - Neurons, Afferent/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Schwann Cells/*physiology
MH  - Tissue Distribution
PMC - PMC6672611
EDAT- 2007/07/13 09:00
MHDA- 2007/08/08 09:00
PMCR- 2008/01/11
CRDT- 2007/07/13 09:00
PHST- 2007/07/13 09:00 [pubmed]
PHST- 2007/08/08 09:00 [medline]
PHST- 2007/07/13 09:00 [entrez]
PHST- 2008/01/11 00:00 [pmc-release]
AID - 27/28/7597 [pii]
AID - 3244300 [pii]
AID - 10.1523/JNEUROSCI.0563-07.2007 [doi]
PST - ppublish
SO  - J Neurosci. 2007 Jul 11;27(28):7597-603. doi: 10.1523/JNEUROSCI.0563-07.2007.