PMID- 17627643
OWN - NLM
STAT- MEDLINE
DCOM- 20071009
LR  - 20151119
IS  - 0105-1873 (Print)
IS  - 0105-1873 (Linking)
VI  - 57
IP  - 2
DP  - 2007 Aug
TI  - The local lymph node assay and the assessment of relative potency: status of 
      validation.
PG  - 70-5
AB  - For the prediction of skin sensitization potential, the local lymph node assay 
      (LLNA) is a fully validated alternative to guinea-pig tests. More recently, 
      information from LLNA dose-response analyses has been used to assess the relative 
      potency of skin sensitizing chemicals. These data are then deployed for risk 
      assessment and risk management. In this commentary, the utility and validity of 
      these relative potency measurements are reviewed. It is concluded that the LLNA 
      does provide a valuable assessment of relative sensitizing potency in the form of 
      the estimated concentration of a chemical required to produce a threefold 
      stimulation of draining lymph node cell proliferation compared with concurrent 
      controls (EC3 value) and that all reasonable validation requirements have been 
      addressed successfully. EC3 measurements are reproducible in both intra- and 
      interlaboratory evaluations and are stable over time. It has been shown also, by 
      several independent groups, that EC3 values correlate closely with data on 
      relative human skin sensitization potency. Consequently, the recommendation made 
      here is that LLNA EC3 measurements should now be regarded as a validated method 
      for the determination of the relative potency of skin sensitizing chemicals, a 
      conclusion that has already been reached by a number of independent expert 
      groups.
FAU - Basketter, David A
AU  - Basketter DA
AD  - Unilever Safety and Environmental Assurance Centre, Colworth Park, Sharnbrook, 
      Bedfordshire, UK. david.basketter@unilever.com
FAU - Gerberick, Frank
AU  - Gerberick F
FAU - Kimber, Ian
AU  - Kimber I
LA  - eng
PT  - Journal Article
PT  - Review
PL  - England
TA  - Contact Dermatitis
JT  - Contact dermatitis
JID - 7604950
RN  - 0 (Allergens)
SB  - IM
MH  - Allergens/pharmacology
MH  - Dermatitis, Allergic Contact/*diagnosis
MH  - Dose-Response Relationship, Drug
MH  - Humans
MH  - *Local Lymph Node Assay
MH  - Reproducibility of Results
MH  - Skin/drug effects
RF  - 53
EDAT- 2007/07/14 09:00
MHDA- 2007/10/10 09:00
CRDT- 2007/07/14 09:00
PHST- 2007/07/14 09:00 [pubmed]
PHST- 2007/10/10 09:00 [medline]
PHST- 2007/07/14 09:00 [entrez]
AID - COD1141 [pii]
AID - 10.1111/j.1600-0536.2007.01141.x [doi]
PST - ppublish
SO  - Contact Dermatitis. 2007 Aug;57(2):70-5. doi: 10.1111/j.1600-0536.2007.01141.x.