PMID- 17628371
OWN - NLM
STAT- MEDLINE
DCOM- 20071018
LR  - 20201209
IS  - 1872-6283 (Electronic)
IS  - 0379-0738 (Linking)
VI  - 170
IP  - 2-3
DP  - 2007 Aug 6
TI  - Evaluation of the IDS One-Step ELISA kits for the detection of illicit drugs in 
      hair.
PG  - 189-92
AB  - This work presents the validation of a new immunological assay, the One-Step 
      enzyme-linked immunosorbent assay (ELISA) tests from International Diagnostic 
      Systems Corp. for the screening of drugs of abuse (cannabis, amphetamines, 
      opiates, and cocaine) in human hair, with subsequent GC-MS confirmation. After 
      decontamination and segmentation into small pieces, 50 mg of hair sample were 
      incubated in 1 ml of methanol during 16 h at 40 degrees C. A 100 microL aliquot 
      was collected and evaporated to dryness in presence of 100 microL of 
      methanol/hydrochloric acid (99:1, v/v) to avoid amphetamines loss. The dried 
      extract was dissolved in 100 microL of the "sample and standard diluent" solution 
      included in the kit. This solution was submitted to analysis according to the 
      recommended instructions of the manufacturer. During the validation phase, GC-MS 
      confirmations were conducted according to our fully validated and published 
      methods for opiates, cocaine, cannabis, and amphetamines determinations in hair. 
      In a last development step, these procedures were slightly modified to directly 
      confirm ELISA results by GC-MS using the methanolic extract. Ninety-three 
      specimens were simultaneously screened by the ELISA tests (103 for 
      tetrahydrocannabinol (THC)) and confirmed by GC-MS. Twenty were found positive 
      for cannabis (THC: 0.10-6.50 ng/mg), 21 for cocaine (0.50-55.20 ng/mg), 24 for 
      opiates (6-acetylmorphine (6-AM): 0.20-11.60 ng/mg, MOR: 0.20-8.90 ng/mg, codeine 
      (COD): 0.20-5.90 ng/mg), and 13 for amphetamines (AP: 0.20 and 0.27 ng/mg, 
      methamphetamine (MAP): 0.30 and 1.10 ng/mg, methylenedioxymethamphetamine (MDMA): 
      0.22-17.80 ng/mg). No false negative results were observed according to the 
      Society of Hair Testing's (SoHT) cutoffs (0.5 ng/mg for cocaine, 0.2 ng/mg for 
      opiates and amphetamines, and 0.1 ng/mg for THC). The One-Step ELISA kits appear 
      suitable due to their sensitivity and specificity for drug of abuse screening in 
      hair. This technology should find interest in workplace drug testing or driving 
      license regranting, especially when many samples have to be tested with a high 
      rate of negative samples, as ELISA is an easy and high-throughput method.
FAU - Pujol, Marie-Laure
AU  - Pujol ML
AD  - Laboratoire ChemTox, Illkirch, France.
FAU - Cirimele, Vincent
AU  - Cirimele V
FAU - Tritsch, Pierre Julien
AU  - Tritsch PJ
FAU - Villain, Marion
AU  - Villain M
FAU - Kintz, Pascal
AU  - Kintz P
LA  - eng
PT  - Journal Article
PT  - Validation Study
DEP - 20070712
PL  - Ireland
TA  - Forensic Sci Int
JT  - Forensic science international
JID - 7902034
RN  - 0 (Amphetamines)
RN  - 0 (Cannabinoids)
RN  - 0 (Dopamine Uptake Inhibitors)
RN  - 0 (Illicit Drugs)
RN  - 0 (Narcotics)
RN  - I5Y540LHVR (Cocaine)
RN  - UX6OWY2V7J (Codeine)
SB  - IM
MH  - Amphetamines/analysis
MH  - Cannabinoids/analysis
MH  - Cocaine/analysis
MH  - Codeine/analysis
MH  - Dopamine Uptake Inhibitors/analysis
MH  - *Enzyme-Linked Immunosorbent Assay
MH  - Forensic Toxicology
MH  - Gas Chromatography-Mass Spectrometry
MH  - Hair/*chemistry
MH  - Humans
MH  - Illicit Drugs/*analysis
MH  - Narcotics/analysis
MH  - Reproducibility of Results
MH  - Substance Abuse Detection/*instrumentation
EDAT- 2007/07/14 09:00
MHDA- 2007/10/19 09:00
CRDT- 2007/07/14 09:00
PHST- 2006/09/01 00:00 [received]
PHST- 2007/02/15 00:00 [revised]
PHST- 2007/02/16 00:00 [accepted]
PHST- 2007/07/14 09:00 [pubmed]
PHST- 2007/10/19 09:00 [medline]
PHST- 2007/07/14 09:00 [entrez]
AID - S0379-0738(07)00549-X [pii]
AID - 10.1016/j.forsciint.2007.02.032 [doi]
PST - ppublish
SO  - Forensic Sci Int. 2007 Aug 6;170(2-3):189-92. doi: 
      10.1016/j.forsciint.2007.02.032. Epub 2007 Jul 12.