PMID- 17629411
OWN - NLM
STAT- MEDLINE
DCOM- 20071214
LR  - 20121115
IS  - 0306-4522 (Print)
IS  - 0306-4522 (Linking)
VI  - 148
IP  - 1
DP  - 2007 Aug 10
TI  - Survival and axonal regeneration of off-center retinal ganglion cells of adult 
      cats are promoted with an anti-glaucoma drug, nipradilol, but not BDNF and CNTF.
PG  - 53-64
AB  - OFF-center retinal ganglion cells (RGCs) occupy a smaller proportion than ON RGCs 
      when RGCs regenerate axons into a transplanted peripheral nerve. We examined 
      whether the regeneration ability of OFF RGCs in adult cats was promoted when the 
      numbers of regenerating RGCs were increased with brain-derived neurotrophic 
      factor (BDNF)+ciliary neurotrophic factor (CNTF)+forskolin (BCF) or 
      3,4-dihydro-8-(2-hydroxy-3-isopropylamino)-propoxy-3-nitroxy-2H-1-benzopyran 
      (nipradilol), an anti-glaucoma drug. ON or OFF RGCs were morphologically 
      determined on the basis of their dendritic ramification in the inner plexiform 
      layer using computational analysis. In the normal intact retina the ratio of ON 
      and OFF RGCs (ON/OFF ratio) was 1.25 (55%/44%); whereas, it was 2.61 in 
      regenerating RGCs with saline injection (control) 6 weeks after peripheral nerve 
      transplantation. Estimated numbers of regenerating ON and OFF RGCs were 2149 and 
      895, respectively. An injection of BCF increased only numbers of ON RGCs into 
      5766 (2.7-fold to control) but not that of OFF RGCs, n=858. Nipradilol increased 
      both estimated numbers of ON (11,518, 5.4-fold to control) and OFF RGCs (7330, 
      8.2-fold to control). In the retinas with optic nerve (OpN) transection and 
      intravitreal saline-, BCF- or nipradilol-injection, numbers of ON and OFF RGCs 
      surviving axotomy showed similar trend to that in regenerating RGCs. Thus, 
      nipradilol promoted the survival and regeneration abilities of both of ON and OFF 
      RGCs whereas BCF only did the abilities of ON RGCs. The distribution of 
      tropo-myosin-related kinase B, BDNF receptor, was sparser in the outer two thirds 
      of inner plexiform layer. The lower surviving ability of OFF-RGCs may be 
      attributed in part to the distribution.
FAU - Yata, T
AU  - Yata T
AD  - Department of Ophthalmology, Nagoya University Graduate School of Medicine, 
      Turuma-cho 65, Showaku, Nagoya, Aichi 466-8550, Japan.
FAU - Nakamura, M
AU  - Nakamura M
FAU - Sagawa, H
AU  - Sagawa H
FAU - Tokita, Y
AU  - Tokita Y
FAU - Terasaki, H
AU  - Terasaki H
FAU - Watanabe, M
AU  - Watanabe M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070712
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - 0 (Antihypertensive Agents)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Ciliary Neurotrophic Factor)
RN  - 0 (Ciliary Neurotrophic Factor Receptor alpha Subunit)
RN  - 0 (Propanolamines)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - FVM336I71Y (nipradilol)
SB  - IM
MH  - Age Factors
MH  - Animals
MH  - Antihypertensive Agents/pharmacology
MH  - Axons/*drug effects/metabolism
MH  - Brain-Derived Neurotrophic Factor/metabolism/*pharmacology/therapeutic use
MH  - Cats
MH  - Cell Count
MH  - Cell Shape/drug effects/physiology
MH  - Cell Survival/drug effects/physiology
MH  - Ciliary Neurotrophic Factor/metabolism/*pharmacology/therapeutic use
MH  - Ciliary Neurotrophic Factor Receptor alpha Subunit/drug effects/metabolism
MH  - Dendrites/ultrastructure
MH  - Denervation
MH  - Glaucoma/drug therapy
MH  - Graft Survival/drug effects/physiology
MH  - Nerve Regeneration/*drug effects/physiology
MH  - Optic Nerve Injuries/metabolism/physiopathology
MH  - Propanolamines/*pharmacology
MH  - Receptor, trkB/drug effects/metabolism
MH  - Recovery of Function/drug effects/physiology
MH  - Retinal Ganglion Cells/cytology/*drug effects/metabolism
EDAT- 2007/07/17 09:00
MHDA- 2007/12/15 09:00
CRDT- 2007/07/17 09:00
PHST- 2007/01/07 00:00 [received]
PHST- 2007/05/14 00:00 [revised]
PHST- 2007/05/18 00:00 [accepted]
PHST- 2007/07/17 09:00 [pubmed]
PHST- 2007/12/15 09:00 [medline]
PHST- 2007/07/17 09:00 [entrez]
AID - S0306-4522(07)00659-8 [pii]
AID - 10.1016/j.neuroscience.2007.05.042 [doi]
PST - ppublish
SO  - Neuroscience. 2007 Aug 10;148(1):53-64. doi: 10.1016/j.neuroscience.2007.05.042. 
      Epub 2007 Jul 12.