PMID- 1763059
OWN - NLM
STAT- MEDLINE
DCOM- 19920207
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 88
IP  - 24
DP  - 1991 Dec 15
TI  - Interaction of the human T-cell lymphotrophic virus type I (HTLV-I) 
      transcriptional activator Tax with cellular factors that bind specifically to the 
      21-base-pair repeats in the HTLV-I enhancer.
PG  - 11445-9
AB  - The human T-cell lymphotrophic virus type I (HTLV-I) Tax protein activates 
      transcription from three 21-base-pair (bp) repeat sequences in the viral 
      enhancer. Using gel electrophoretic mobility-shift assays, we now show that Tax 
      interacts directly with the nuclear proteins, Tax activation factors (TAFs), that 
      bind the 21-bp repeats. This interaction is demonstrated by decreased 
      electrophoretic mobilities of the TAFs-21-bp-repeats complexes upon supply of Tax 
      exogenously. Formation of the TAFs-21-bp-repeats and Tax-TAFs-21-bp-repeats 
      complexes correlates with in vivo transactivation by Tax. Furthermore, 
      interaction of Tax with TAFs enhances their binding to the 21-bp repeats. These 
      data indicate that trans-activation by Tax is most likely mediated by interaction 
      of Tax with TAFs.
FAU - Zhao, L J
AU  - Zhao LJ
AD  - Department of Medicine, Case Western Reserve University School of Medicine, 
      Cleveland, OH 44106.
FAU - Giam, C Z
AU  - Giam CZ
LA  - eng
GR  - R01 CA48709/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Gene Products, tax)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 0 (Recombinant Fusion Proteins)
SB  - IM
MH  - Base Composition
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - DNA-Binding Proteins/*metabolism
MH  - *Enhancer Elements, Genetic
MH  - Escherichia coli/genetics
MH  - Gene Products, tax/*genetics/*metabolism
MH  - Human T-lymphotropic virus 1/genetics/*metabolism
MH  - Humans
MH  - Molecular Sequence Data
MH  - Mutagenesis, Site-Directed
MH  - Oligodeoxyribonucleotides
MH  - Plasmids
MH  - Polymerase Chain Reaction/methods
MH  - Recombinant Fusion Proteins/metabolism
MH  - *Repetitive Sequences, Nucleic Acid
MH  - Restriction Mapping
MH  - Transcription, Genetic
PMC - PMC53152
EDAT- 1991/12/15 00:00
MHDA- 1991/12/15 00:01
PMCR- 1992/06/15
CRDT- 1991/12/15 00:00
PHST- 1991/12/15 00:00 [pubmed]
PHST- 1991/12/15 00:01 [medline]
PHST- 1991/12/15 00:00 [entrez]
PHST- 1992/06/15 00:00 [pmc-release]
AID - 10.1073/pnas.88.24.11445 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1991 Dec 15;88(24):11445-9. doi: 
      10.1073/pnas.88.24.11445.