PMID- 17631102
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20070907
LR  - 20070716
IS  - 1873-4324 (Electronic)
IS  - 0003-2670 (Linking)
VI  - 596
IP  - 2
DP  - 2007 Jul 23
TI  - High-performance liquid chromatographic/ion-trap mass spectrometric speciation of 
      aquatic mercury as its pyrrolidinedithiocarbamate complexes.
PG  - 242-50
AB  - Mercury-pyrrolidinedithiocarbamate complexes are first time used for speciation 
      of aquatic mercury with high-performance liquid chromatographic/ion trap-mass 
      spectrometric method utilizing atmospheric pressure chemical ionization (APCI). 
      The separation of the four mercury species was achieved in less than 5 min with a 
      linear gradient profile of aqueous methanol from 70 up to 100% (v/v) in 4th min, 
      isocratic elution at 100% up to 5th min and followed by a negative gradient to 
      70% in 6th min. The best separation was achieved on a reverse phase Zorbax 
      Eclipse XDB C18 column (50 mm x 2.1 mm i.d., 1.8 microm particle size). The 
      on-column limits of detection (injection volume 1 microL) were 370 pg for 
      methylmercury (MeHg+), 280 pg for ethylmercury (EtHg+), 250 pg for phenylmercury 
      (PhHg+) and 90 pg for inorganic mercury (Hg2+) when the data were collected in 
      selective ion monitoring (SIM) mode. A method of isolation and preconcentration 
      of the mercury species using a "home-made" C18 solid phase extraction (SPE) 
      microcolumns was developed to enhance sensitivity of the method. The 
      preconcentration factor as much as 2500 was achieved with on-column complex 
      formation of mercury-pyrrolidinedithiocarbamate. Methanol (100%) was chosen for 
      elution of preconcentrated mercury species. The method was applied for the 
      determination of mercury species in river water samples.
FAU - Houserova, Pavlina
AU  - Houserova P
AD  - Department of Chemistry and Biochemistry, Mendel University of Agriculture and 
      Forestry, Zemedelska 1, Brno, CZ 613 00, Czech Republic.
FAU - Matejicek, David
AU  - Matejicek D
FAU - Kuban, Vlastimil
AU  - Kuban V
LA  - eng
PT  - Journal Article
DEP - 20070616
PL  - Netherlands
TA  - Anal Chim Acta
JT  - Analytica chimica acta
JID - 0370534
EDAT- 2007/07/17 09:00
MHDA- 2007/07/17 09:01
CRDT- 2007/07/17 09:00
PHST- 2007/04/05 00:00 [received]
PHST- 2007/06/06 00:00 [revised]
PHST- 2007/06/10 00:00 [accepted]
PHST- 2007/07/17 09:00 [pubmed]
PHST- 2007/07/17 09:01 [medline]
PHST- 2007/07/17 09:00 [entrez]
AID - S0003-2670(07)01049-5 [pii]
AID - 10.1016/j.aca.2007.06.020 [doi]
PST - ppublish
SO  - Anal Chim Acta. 2007 Jul 23;596(2):242-50. doi: 10.1016/j.aca.2007.06.020. Epub 
      2007 Jun 16.