PMID- 17632455
OWN - NLM
STAT- MEDLINE
DCOM- 20071025
LR  - 20230210
IS  - 0893-3952 (Print)
IS  - 0893-3952 (Linking)
VI  - 20
IP  - 9
DP  - 2007 Sep
TI  - Gene fusions between TMPRSS2 and ETS family genes in prostate cancer: frequency 
      and transcript variant analysis by RT-PCR and FISH on paraffin-embedded tissues.
PG  - 921-8
AB  - Recurrent gene fusions between TMPRSS2 and ETS family genes have recently been 
      shown to occur at a high frequency in prostate cancer. In this study, we used 
      formalin-fixed paraffin-embedded tissue and evaluated both TMPRSS2-ERG and 
      TMPRSS2-ETV1 fusions by reverse transcription polymerase chain reaction (RT-PCR) 
      and fluorescence in situ hybridization (FISH). The results were correlated to 
      overexpression of the downstream ERG and ETV1 sequences. Of 82 cases examined, 
      TMPRSS2-ETV1 fusion was seen in only one case, by FISH. In comparison, 
      TMPRSS2-ERG fusion was documented in 35 cases (43%) by either RT-PCR or FISH. 
      Deletion, rather than translocation, was found to be the main mechanism for 
      TMPRSS2-ERG gene fusion (81 vs 19%). RT-PCR and FISH results correlated well, 
      with most positive cases resulting in overexpression of downstream ERG sequences. 
      Several TMPRSS2-ERG fusion transcript variants were identified, most of which are 
      predicted to encode truncated ERG proteins. Prostate cancer of Gleason's scores 6 
      or 7 had more frequent TMPRSS2-ERG fusions than higher-grade tumors, but this 
      difference was not statistically significant (P=0.42). On the other hand, 
      mucin-positive carcinomas more often harbor such gene fusions when compared to 
      mucin-negative tumors (P=0.004). These morphological correlates, and more 
      importantly the potential correlation of such fusions to clinical outcome and 
      treatment responses, should be further explored.
FAU - Tu, Jiangling J
AU  - Tu JJ
AD  - Department of Pathology and Laboratory Medicine, Weill Cornell Medical College, 
      New York, NY 10021, USA.
FAU - Rohan, Stephen
AU  - Rohan S
FAU - Kao, Jean
AU  - Kao J
FAU - Kitabayashi, Naoki
AU  - Kitabayashi N
FAU - Mathew, Susan
AU  - Mathew S
FAU - Chen, Yao-Tseng
AU  - Chen YT
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20070713
PL  - United States
TA  - Mod Pathol
JT  - Modern pathology : an official journal of the United States and Canadian Academy 
      of Pathology, Inc
JID - 8806605
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (ERG protein, human)
RN  - 0 (ETV1 protein, human)
RN  - 0 (Oncogene Proteins, Fusion)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
RN  - 0 (Transcriptional Regulator ERG)
RN  - 63231-63-0 (RNA)
RN  - EC 3.4.21.- (Serine Endopeptidases)
RN  - EC 3.4.21.- (TMPRSS2 protein, human)
SB  - IM
MH  - DNA-Binding Proteins/*genetics
MH  - Gene Deletion
MH  - Gene Expression Regulation, Neoplastic
MH  - *Gene Fusion
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Male
MH  - Neoplasm Staging
MH  - Oncogene Proteins, Fusion/*genetics
MH  - Paraffin Embedding
MH  - Prostatic Neoplasms/*genetics/pathology
MH  - RNA/analysis
MH  - *Reverse Transcriptase Polymerase Chain Reaction
MH  - Serine Endopeptidases/*genetics
MH  - Trans-Activators/*genetics
MH  - Transcription Factors/*genetics
MH  - Transcriptional Regulator ERG
MH  - Translocation, Genetic
EDAT- 2007/07/17 09:00
MHDA- 2007/10/27 09:00
CRDT- 2007/07/17 09:00
PHST- 2007/07/17 09:00 [pubmed]
PHST- 2007/10/27 09:00 [medline]
PHST- 2007/07/17 09:00 [entrez]
AID - S0893-3952(22)03265-3 [pii]
AID - 10.1038/modpathol.3800903 [doi]
PST - ppublish
SO  - Mod Pathol. 2007 Sep;20(9):921-8. doi: 10.1038/modpathol.3800903. Epub 2007 Jul 
      13.