PMID- 17633185
OWN - NLM
STAT- MEDLINE
DCOM- 20080702
LR  - 20161018
IS  - 0250-3301 (Print)
IS  - 0250-3301 (Linking)
VI  - 28
IP  - 5
DP  - 2007 May
TI  - [Bacterial diversity in sequencing batch biofilm reactor (SBBR) for landfill 
      leachate treatment using PCR-DGGE].
PG  - 1095-101
AB  - For studying the bacterial diversity and the mechanism of denitrification in 
      sequencing bath biofilm reactor (SBBR) treating landfill leachate to provide 
      microbial evidence for technique improvements, total microbial DNA was extracted 
      from samples which were collected from natural landfill leachate and biofilm of a 
      SBBR that could efficiently remove NH4+ -N and COD of high concentration. 16S 
      rDNA fragments were amplified from the total DNA successfully using a pair of 
      universal bacterial 16S rDNA primer, GC341F and 907R, and then were used for 
      denaturing gradient gel electrophoresis (DGGE) analysis. The bands in the gel 
      were analyzed by statistical methods and excided from the gel for sequencing, and 
      the sequences were used for homology analysis and then two phylogenetic trees 
      were constructed using DNAStar software. Results indicated that the bacterial 
      diversity of the biofilm in SBBR and the landfill leachate was abundant, and no 
      obvious change of community structure happened during running in the biofilm, in 
      which most bacteria came from the landfill leachate. There may be three different 
      modes of denitrification in the reactor because several different nitrifying 
      bacteria, denitrifying bacteria and anaerobic ammonia oxidation bacteria 
      coexisted in it. The results provided some valuable references for studying 
      microbiological mechanism of denitrification in SBBR.
FAU - Xiao, Yong
AU  - Xiao Y
AD  - College of Environmental Science and Engineering, Hunan University, Changsha 
      410082, China. xiaoyong1982@msn.com
FAU - Yang, Zhao-hui
AU  - Yang ZH
FAU - Zeng, Guang-ming
AU  - Zeng GM
FAU - Ma, Yan-he
AU  - Ma YH
FAU - Liu, You-sheng
AU  - Liu YS
FAU - Wang, Rong-juan
AU  - Wang RJ
FAU - Xu, Zheng-yong
AU  - Xu ZY
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Huan Jing Ke Xue
JT  - Huan jing ke xue= Huanjing kexue
JID - 8405344
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sewage)
SB  - IM
MH  - Bacteria/classification/genetics/*growth & development
MH  - Biodiversity
MH  - *Biofilms
MH  - Bioreactors/*microbiology
MH  - Electrophoresis, Agar Gel/methods
MH  - Environmental Microbiology
MH  - Genetic Variation
MH  - Phylogeny
MH  - Polymerase Chain Reaction/methods
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sewage/*microbiology
MH  - Waste Disposal, Fluid/*methods
EDAT- 2007/07/18 09:00
MHDA- 2008/07/03 09:00
CRDT- 2007/07/18 09:00
PHST- 2007/07/18 09:00 [pubmed]
PHST- 2008/07/03 09:00 [medline]
PHST- 2007/07/18 09:00 [entrez]
PST - ppublish
SO  - Huan Jing Ke Xue. 2007 May;28(5):1095-101.