PMID- 17634295
OWN - NLM
STAT- MEDLINE
DCOM- 20071106
LR  - 20181113
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 45
IP  - 9
DP  - 2007 Sep
TI  - Molecular characterization of vancomycin-resistant enterococcus faecium isolates 
      from mainland China.
PG  - 2813-8
AB  - Little is known about vancomycin-resistant enterococci in China. Thirteen 
      pulsed-field gel electrophoresis-confirmed heterogeneous VanA-type 
      vancomycin-resistant Enterococcus faecium (VRE) isolates were obtained from five 
      Chinese hospitals from 2001 to 2005. The isolates were typed by multilocus 
      sequence typing into nine different sequence types (STs), including five new STs 
      (ST18, ST25, ST78, ST203, ST320, ST321, ST322, ST323, and ST335). Vancomycin 
      resistance in each isolate was encoded on conjugative plasmids; two of the 
      plasmids, pZB18 (67 kbp) and pZB22 (200 kbp), were highly conjugative and were 
      able to transfer at high frequencies of around 10(-4) and 10(-7) per donor cell 
      in broth mating, respectively. None of the plasmids identified in these isolates 
      carried traA, which is usually conserved in the pMG1-like highly conjugative 
      plasmid for E. faecium, implying that pZB18 and pZB22 were novel types of a 
      highly conjugative plasmid in enterococci. Thirteen Tn1546-like elements encoding 
      VanA-type VRE on the conjugative plasmids were classified into six types (types I 
      to VI), and most of them contained both IS1216V and IS1542 insertions. The 
      isolates carrying the type II element were predominant. The six type elements 
      were different from that of a VanA-type Enterococcus faecalis strain isolated 
      from Chinese chicken meat. The results suggested that the disseminations of VRE 
      in these areas were by Tn1546-like elements being acquired by the conjugative 
      plasmids and transferred among E. faecium strains.
FAU - Zheng, Bo
AU  - Zheng B
AD  - Department of Bacteriology and Bacterial Infection Control, Gunma University 
      Graduate School of Medicine, Maebashi, Gunma 371-8511, Japan.
FAU - Tomita, Haruyoshi
AU  - Tomita H
FAU - Xiao, Yong Hong
AU  - Xiao YH
FAU - Wang, Shan
AU  - Wang S
FAU - Li, Yun
AU  - Li Y
FAU - Ike, Yasuyoshi
AU  - Ike Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070718
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (DNA Transposable Elements)
RN  - 0 (DNA, Bacterial)
SB  - IM
MH  - Bacterial Typing Techniques/methods
MH  - China/epidemiology
MH  - Conjugation, Genetic
MH  - DNA Transposable Elements/genetics
MH  - DNA, Bacterial/chemistry/genetics
MH  - Enterococcus faecium/*classification/drug effects/*genetics/isolation & 
      purification
MH  - Gene Transfer, Horizontal
MH  - Genotype
MH  - Gram-Positive Bacterial Infections/epidemiology/*microbiology
MH  - Humans
MH  - Molecular Epidemiology
MH  - Plasmids/genetics
MH  - Sequence Analysis, DNA
MH  - Vancomycin Resistance/*genetics
PMC - PMC2045241
EDAT- 2007/07/20 09:00
MHDA- 2007/11/07 09:00
PMCR- 2008/01/01
CRDT- 2007/07/20 09:00
PHST- 2007/07/20 09:00 [pubmed]
PHST- 2007/11/07 09:00 [medline]
PHST- 2007/07/20 09:00 [entrez]
PHST- 2008/01/01 00:00 [pmc-release]
AID - JCM.00457-07 [pii]
AID - 0457-07 [pii]
AID - 10.1128/JCM.00457-07 [doi]
PST - ppublish
SO  - J Clin Microbiol. 2007 Sep;45(9):2813-8. doi: 10.1128/JCM.00457-07. Epub 2007 Jul 
      18.