PMID- 17635552
OWN - NLM
STAT- MEDLINE
DCOM- 20070913
LR  - 20220408
IS  - 1462-2912 (Print)
IS  - 1462-2912 (Linking)
VI  - 9
IP  - 8
DP  - 2007 Aug
TI  - Fluorescence in situ hybridization analysis of hindgut bacteria associated with 
      the development of equine laminitis.
PG  - 2090-100
AB  - Carbohydrate-induced laminitis in horses is characterized by marked changes in 
      the composition of the hindgut microbiota, from a predominantly Gram-negative 
      population to one dominated by Gram-positive bacteria. The objective of this 
      study was to monitor changes in the relative abundance of selected hindgut 
      bacteria that have previously been implicated in the pathophysiology of equine 
      laminitis using fluorescence in situ hybridization (FISH). Caecal cannulae were 
      surgically implanted in five Standardbred horses and laminitis induced by oral 
      administration of a bolus dose of oligofructose. Caecal fluid and faecal 
      specimens were collected over a 48 h period at 2 to 4 h intervals 
      post-oligofructose administration and subjected to FISH using probes specific for 
      nine bacterial groups to determine changes in their relative abundance compared 
      with total bacteria hybridizing to the generic EUBMIX probe. Additionally, hoof 
      biopsies were taken over the course of the experiment at 6 h intervals and 
      evaluated for histopathological changes consistent with laminitis, allowing 
      changes in hindgut microbiota to be correlated with the onset of lesions in the 
      foot. Of the microorganisms specifically targeted, streptococci of the 
      Streptococcus bovis/equinus complex were the only bacteria that consistently 
      proliferated in both caecal fluid and faeces immediately before the onset of 
      histological signs of laminitis. Furthermore, lactobacilli, Enterobacteriaceae, 
      Allisonella histaminiformans, enterococci, Bacteroides fragilis, Mitsuokella 
      jalaludinii and Clostridium difficile did not establish significant populations 
      in the hindgut before the onset of equine laminitis.
FAU - Milinovich, Gabriel J
AU  - Milinovich GJ
AD  - Australian Equine Laminitis Research Unit, School of Veterinary Science, The 
      University of Queensland, Brisbane, Qld, Australia. g.milinovich@uq.edu.au
FAU - Trott, Darren J
AU  - Trott DJ
FAU - Burrell, Paul C
AU  - Burrell PC
FAU - Croser, Emma L
AU  - Croser EL
FAU - Al Jassim, Rafat A M
AU  - Al Jassim RA
FAU - Morton, John M
AU  - Morton JM
FAU - van Eps, Andrew W
AU  - van Eps AW
FAU - Pollitt, Christopher C
AU  - Pollitt CC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Environ Microbiol
JT  - Environmental microbiology
JID - 100883692
RN  - 0 (Oligosaccharides)
RN  - 0 (oligofructose)
SB  - IM
MH  - Animals
MH  - Bacteria/classification/*isolation & purification
MH  - Cecum/*microbiology
MH  - Feces/microbiology
MH  - Foot Diseases/chemically induced/microbiology/pathology/*veterinary
MH  - Hoof and Claw/pathology
MH  - Horse Diseases/chemically induced/*microbiology/pathology
MH  - Horses
MH  - In Situ Hybridization, Fluorescence
MH  - Oligosaccharides
EDAT- 2007/07/20 09:00
MHDA- 2007/09/14 09:00
CRDT- 2007/07/20 09:00
PHST- 2007/07/20 09:00 [pubmed]
PHST- 2007/09/14 09:00 [medline]
PHST- 2007/07/20 09:00 [entrez]
AID - EMI1327 [pii]
AID - 10.1111/j.1462-2920.2007.01327.x [doi]
PST - ppublish
SO  - Environ Microbiol. 2007 Aug;9(8):2090-100. doi: 10.1111/j.1462-2920.2007.01327.x.