PMID- 17635597
OWN - NLM
STAT- MEDLINE
DCOM- 20070918
LR  - 20090202
IS  - 0017-8748 (Print)
IS  - 0017-8748 (Linking)
VI  - 47
IP  - 7
DP  - 2007 Jul-Aug
TI  - Association between a polymorphism in the lymphotoxin-a promoter region and 
      migraine.
PG  - 1056-62
AB  - OBJECTIVE: The aim of this study was to determine whether polymorphisms in the 
      lymphotoxin (LTA)-tumor necrosis factor (TNF) region are associated with the risk 
      of migraine. BACKGROUND: Previous studies concerning the role of TNFalpha in 
      migraine have provided conflicting results. It has been reported that LTA could 
      be a susceptibility gene in migraine. It is possible that the TNFalpha 
      polymorphism associated with migraine is in linkage disequilibrium with other 
      functional polymorphisms that influence migraine risk. Moreover, there are 
      significant differences among the allele frequencies of TNF gene variants among 
      populations from different ethnic groups. METHODS: In a case-control study, 
      including 439 migraine patients and 382 controls, we examined the association 
      between 15 single nucleotide polymorphisms in the coding and promoter regions of 
      LTA and TNFalpha genes, which are located within the 22 kb around TNF and the 
      risk of migraine. We performed a chromatin immunoprecipitation (ChIP) assay and 
      an electrophoretic mobility shift assay (EMSA) to identify differential 
      protein-DNA binding of LTA-294. RESULTS: Homozygosity for the LTA-294C allele was 
      significantly associated with an increased risk of migraine compared with CT/TT 
      carriers (corrected P= .005, odds ratio [OR]= 1.7, 95% confidence interval [CI] 
      1.3-2.3). Haplotype TGAAC was found to be significantly associated with a 
      protective effect against migraine (P= .0005, Bonferroni corrected P= .003, OR = 
      0.7, 95% CI 0.5-0.8). There was no differential protein-DNA binding pattern in 
      both EMSA and ChIP assays. CONCLUSIONS: We found that the LTA haplotypes were 
      associated with migraine among Koreans and that the best marker for this is the 
      LTA-294 T/C polymorphism. Our results indicate that these associations should be 
      defined in the context of the involvement of other genetically linked region, 
      such as human leukocyte antigen (HLA) loci.
FAU - Lee, Kyung-A
AU  - Lee KA
AD  - Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, 
      South Korea.
FAU - Jang, Soo Yeon
AU  - Jang SY
FAU - Sohn, Kwang-Min
AU  - Sohn KM
FAU - Won, Hong Hee
AU  - Won HH
FAU - Kim, Min Ji
AU  - Kim MJ
FAU - Kim, Jong-Won
AU  - Kim JW
FAU - Chung, Chin-Sang
AU  - Chung CS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Headache
JT  - Headache
JID - 2985091R
RN  - 0 (Genetic Markers)
RN  - 0 (Lymphotoxin-alpha)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - DNA/genetics/isolation & purification
MH  - Electrophoretic Mobility Shift Assay
MH  - Genetic Markers
MH  - Genotype
MH  - Humans
MH  - Lymphotoxin-alpha/blood/*genetics
MH  - Middle Aged
MH  - Migraine Disorders/blood/*genetics
MH  - *Polymorphism, Genetic
MH  - *Polymorphism, Single Nucleotide
MH  - Promoter Regions, Genetic
MH  - Reference Values
MH  - Tumor Necrosis Factor-alpha/*genetics
EDAT- 2007/07/20 09:00
MHDA- 2007/09/19 09:00
CRDT- 2007/07/20 09:00
PHST- 2007/07/20 09:00 [pubmed]
PHST- 2007/09/19 09:00 [medline]
PHST- 2007/07/20 09:00 [entrez]
AID - HED847 [pii]
AID - 10.1111/j.1526-4610.2007.00847.x [doi]
PST - ppublish
SO  - Headache. 2007 Jul-Aug;47(7):1056-62. doi: 10.1111/j.1526-4610.2007.00847.x.