PMID- 17635654
OWN - NLM
STAT- MEDLINE
DCOM- 20070829
LR  - 20070719
IS  - 0022-2720 (Print)
IS  - 0022-2720 (Linking)
VI  - 227
IP  - Pt 1
DP  - 2007 Jul
TI  - Centrifugation of bacterial cells on slide surface improves the spatial 
      distribution, cell recovery and reduces the time of enumeration for fluorescence 
      in situ hybridization.
PG  - 8-14
AB  - Fluorescence in situ hybridization (FISH), which is used for the enumeration of 
      bacteria in various ecosystems including the human intestinal tract, has several 
      limitations. One of the major problems encountered is the uneven distribution of 
      bacterial cells on the slide surface, which increases the coefficient of 
      variation between repetitions and thereby increase the time required for 
      enumeration. In order to improve the spatial distribution, we designed a 
      centrifugation device, which allows the direct centrifugation of bacterial cells 
      onto the slide surface. Another problem is the loss of bacterial cells during the 
      hybridization procedure. This leads to an underestimation of the true cell 
      numbers in the sample. To overcome this problem we tested the use of silanized or 
      chrome gelatine coated slides. Our study indicates that the use of the 
      centrifugation device in conjunction with chrome gelatine coated slides highly 
      improves the quality of enumeration data obtained by manual and automated 
      microscopic counting and shortens the time of analysis.
FAU - Namsolleck, Pawel
AU  - Namsolleck P
AD  - Department of Gastrointestinal Microbiology, German Institute of Human Nutrition 
      Potsdam-Rehbrucke, Arthur-Scheunert Allee 114-116, 14558 Nuthetal, Germany.
FAU - Mohan, Ruchika
AU  - Mohan R
FAU - Koebnick, Corinna
AU  - Koebnick C
FAU - Blaut, Michael
AU  - Blaut M
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Microsc
JT  - Journal of microscopy
JID - 0204522
SB  - IM
MH  - Analysis of Variance
MH  - *Centrifugation
MH  - Colony Count, Microbial/instrumentation/*methods
MH  - *In Situ Hybridization, Fluorescence
MH  - Reproducibility of Results
EDAT- 2007/07/20 09:00
MHDA- 2007/08/30 09:00
CRDT- 2007/07/20 09:00
PHST- 2007/07/20 09:00 [pubmed]
PHST- 2007/08/30 09:00 [medline]
PHST- 2007/07/20 09:00 [entrez]
AID - JMI1780 [pii]
AID - 10.1111/j.1365-2818.2007.01780.x [doi]
PST - ppublish
SO  - J Microsc. 2007 Jul;227(Pt 1):8-14. doi: 10.1111/j.1365-2818.2007.01780.x.