PMID- 17638388
OWN - NLM
STAT- MEDLINE
DCOM- 20071017
LR  - 20181113
IS  - 1932-8451 (Print)
IS  - 1932-846X (Electronic)
IS  - 1932-8451 (Linking)
VI  - 67
IP  - 10
DP  - 2007 Sep 1
TI  - Neuronal nitric oxide synthase and calbindin delineate sex differences in the 
      developing hypothalamus and preoptic area.
PG  - 1371-81
AB  - Throughout the hypothalamus there are several regions known to contain sex 
      differences in specific cellular, neurochemical, or cell grouping 
      characteristics. The current study examined the potential origin of sex 
      differences in calbindin expression in the preoptic area and hypothalamus as 
      related to sources of nitric oxide. Specific cell populations were defined by 
      immunoreactive (ir) calbindin and neuronal nitric oxide synthase (nNOS) in the 
      preoptic area/anterior hypothalamus (POA/AH), anteroventral periventricular 
      nucleus (AVPv), and ventromedial nucleus of the hypothalamus (VMN). The POA/AH of 
      adult mice was characterized by a striking sex difference in the distribution of 
      cells with ir-calbindin. Examination of the POA/AH of androgen receptor deficient 
      Tfm mice suggests that this pattern was in part androgen receptor dependent, 
      since Tfm males had reduced ir-calbindin compared with wild-type males and more 
      similar to wild-type females. At P0 ir-calbindin was more prevalent than in 
      adulthood, with males having significantly more ir-calbindin and nNOS than have 
      females. Cells that contained either ir-calbindin or ir-nNOS in the POA/AH were 
      in adjacent cell groups, suggesting that NO derived from the enzymatic activity 
      of nNOS may influence the development of ir-calbindin cells. In the region of 
      AVPv, at P0, there was a sex difference with males having more ir-nNOS fibers 
      than have females while ir-calbindin was not detected. In the VMN, at P0, ir-nNOS 
      was greater in females than in males, with no significant difference in 
      ir-calbindin. We suggest that NO as an effector molecule and calbindin as a 
      molecular biomarker illuminate key aspects of sexual differentiation in the 
      developing mouse brain.
FAU - Edelmann, Michelle
AU  - Edelmann M
AD  - Colorado State University, Fort Collins, Colorado 80523, USA.
FAU - Wolfe, Cory
AU  - Wolfe C
FAU - Scordalakes, Elka M
AU  - Scordalakes EM
FAU - Rissman, Emilie F
AU  - Rissman EF
FAU - Tobet, Stuart
AU  - Tobet S
LA  - eng
GR  - MH057759/MH/NIMH NIH HHS/United States
GR  - R01 MH061376/MH/NIMH NIH HHS/United States
GR  - R01 MH057759-02/MH/NIMH NIH HHS/United States
GR  - MH61376/MH/NIMH NIH HHS/United States
GR  - R01 MH057759/MH/NIMH NIH HHS/United States
GR  - R01 MH061376-03/MH/NIMH NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - Dev Neurobiol
JT  - Developmental neurobiology
JID - 101300215
RN  - 0 (Biomarkers)
RN  - 0 (Calbindins)
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Receptors, Androgen)
RN  - 0 (S100 Calcium Binding Protein G)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type I)
RN  - EC 1.14.13.39 (Nos1 protein, mouse)
SB  - IM
MH  - Animals
MH  - Biomarkers/analysis/metabolism
MH  - Calbindins
MH  - Calcium-Binding Proteins/*metabolism
MH  - Female
MH  - Hypothalamus/growth & development/*metabolism
MH  - Immunohistochemistry
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - Nerve Tissue Proteins/*metabolism
MH  - Neurons/metabolism
MH  - Nitric Oxide/biosynthesis
MH  - Nitric Oxide Synthase Type I/*metabolism
MH  - Preoptic Area/growth & development/*metabolism
MH  - Receptors, Androgen/metabolism
MH  - S100 Calcium Binding Protein G
MH  - *Sex Characteristics
MH  - Sex Differentiation/*physiology
MH  - Sexual Maturation/physiology
PMC - PMC3622702
MID - NIHMS341445
EDAT- 2007/07/20 09:00
MHDA- 2007/10/18 09:00
PMCR- 2013/04/11
CRDT- 2007/07/20 09:00
PHST- 2007/07/20 09:00 [pubmed]
PHST- 2007/10/18 09:00 [medline]
PHST- 2007/07/20 09:00 [entrez]
PHST- 2013/04/11 00:00 [pmc-release]
AID - 10.1002/dneu.20507 [doi]
PST - ppublish
SO  - Dev Neurobiol. 2007 Sep 1;67(10):1371-81. doi: 10.1002/dneu.20507.