PMID- 17638662
OWN - NLM
STAT- MEDLINE
DCOM- 20070823
LR  - 20240507
IS  - 0002-9173 (Print)
IS  - 0002-9173 (Linking)
VI  - 128
IP  - 2
DP  - 2007 Aug
TI  - Correlating routine cytology, quantitative nuclear morphometry by digital image 
      analysis, and genetic alterations by fluorescence in situ hybridization to assess 
      the sensitivity of cytology for detecting pancreatobiliary tract malignancy.
PG  - 272-9
AB  - Routine cytologic (RC), fluorescence in situ hybridization (FISH), digital image 
      analysis (DIA), and quantifiable morphometric results from 284 pancreatobiliary 
      stricture brushings were compared. We chose specific DIA nuclear features 
      assessed by pathologists in evaluating RC specimens, such as area and shape. A 
      visual nuclear morphometric score (VNMS) was calculated. There was a difference 
      (P < .001) in the mean VNMS when RC results were classified as negative (11.5), 
      atypical (12.5), suspicious (13.8), and positive (16.5). The mean VNMS of 
      specimens diagnosed as disomy (11.3), trisomy 7 (12.1), and polysomy (14.7) by 
      FISH was also different (P < .001). There was no difference in the VNMS of 
      false-negative and true-negative cytologic specimens (P = .225). Our findings 
      substantiate the relationship between cell nuclear visual alterations and genetic 
      FISH abnormalities. The low sensitivity of cytologic examination for 
      pancreatobiliary carcinoma is due to an absence of tumor cells or the presence of 
      well-differentiated tumor lacking recognizable nuclear atypia.
FAU - Barr Fritcher, Emily G
AU  - Barr Fritcher EG
AD  - Department of Laboratory Medicine and Pathology, Mayo Clinic and Foundation, 
      Rochester, MN, USA.
FAU - Kipp, Benjamin R
AU  - Kipp BR
FAU - Slezak, Jeffrey M
AU  - Slezak JM
FAU - Moreno-Luna, Laura E
AU  - Moreno-Luna LE
FAU - Gores, Gregory J
AU  - Gores GJ
FAU - Levy, Michael J
AU  - Levy MJ
FAU - Roberts, Lewis R
AU  - Roberts LR
FAU - Halling, Kevin C
AU  - Halling KC
FAU - Sebo, Thomas J
AU  - Sebo TJ
LA  - eng
PT  - Journal Article
PL  - England
TA  - Am J Clin Pathol
JT  - American journal of clinical pathology
JID - 0370470
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Bile Duct Neoplasms/genetics/*pathology
MH  - Cell Nucleus/*pathology
MH  - Child
MH  - *Chromosome Aberrations
MH  - Female
MH  - Humans
MH  - Image Processing, Computer-Assisted/*methods
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Middle Aged
MH  - Pancreatic Neoplasms/genetics/*pathology
MH  - Sensitivity and Specificity
MH  - Trisomy
EDAT- 2007/07/20 09:00
MHDA- 2007/08/24 09:00
CRDT- 2007/07/20 09:00
PHST- 2007/07/20 09:00 [pubmed]
PHST- 2007/08/24 09:00 [medline]
PHST- 2007/07/20 09:00 [entrez]
AID - MX425N1820067602 [pii]
AID - 10.1309/BC6DY755Q3T5W9EE [doi]
PST - ppublish
SO  - Am J Clin Pathol. 2007 Aug;128(2):272-9. doi: 10.1309/BC6DY755Q3T5W9EE.