PMID- 17638669
OWN - NLM
STAT- MEDLINE
DCOM- 20070823
LR  - 20091119
IS  - 0002-9173 (Print)
IS  - 0002-9173 (Linking)
VI  - 128
IP  - 2
DP  - 2007 Aug
TI  - Chronic lymphocytic leukemia FISH panel: impact on diagnosis.
PG  - 323-32
AB  - Interphase fluorescence in situ hybridization (FISH) is an alternative to 
      conventional chromosome analysis of chronic lymphocytic leukemia (CLL) cells. We 
      analyzed 172 samples from 136 possible CLL cases using a FISH panel. Reflex 
      testing with probes to CCND1, BCL2, BCL3, BCL11A, c-MYC, MALT1, and a break-apart 
      immunoglobulin heavy chain (IGH) probe was done if more than 2 signals for 14q32 
      occurred. For 111 cases, there were sufficient data for analysis. Of 111 cases, 
      81 (72.9%) had 1 or more genetic abnormalities. The most frequent abnormality was 
      13q-, followed by trisomy 12, 11q-, and 17p-. In 13 cases, there were IGH 
      abnormalities. Two cases with CCND1/IGH fusion were reclassified as mantle cell 
      lymphoma. Four CLL cases had IGH fusion with BCL2, BCL3 (2 cases), and BCL11A; no 
      fusion partner was detected in 7 cases. Morphologic features were atypical for 
      CLL in 2 cases with IGH fusion (BCL11A and BCL3). The FISH CLL panel is useful to 
      identify prognostic aberrations and to clarify diagnosis in cases with unusual 
      morphologic features.
FAU - Nelson, Beverly P
AU  - Nelson BP
AD  - Department of Pathology, Feinberg Medical School, Northwestern University, 
      Chicago, IL 60611-2908, USA.
FAU - Gupta, Rohit
AU  - Gupta R
FAU - Dewald, Gordon W
AU  - Dewald GW
FAU - Paternoster, Sarah F
AU  - Paternoster SF
FAU - Rosen, Steven T
AU  - Rosen ST
FAU - Peterson, LoAnn C
AU  - Peterson LC
LA  - eng
PT  - Journal Article
PL  - England
TA  - Am J Clin Pathol
JT  - American journal of clinical pathology
JID - 0370470
RN  - 0 (Cyclin D)
RN  - 0 (Cyclins)
RN  - 0 (Immunoglobulin Heavy Chains)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Chromosomes, Human, Pair 14
MH  - Cyclin D
MH  - Cyclins/genetics
MH  - Female
MH  - Humans
MH  - Immunoglobulin Heavy Chains/genetics
MH  - Immunophenotyping
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/diagnosis/*genetics/immunology/pathology
MH  - Lymphoma, Mantle-Cell/genetics
MH  - Male
MH  - Middle Aged
MH  - Prognosis
MH  - Translocation, Genetic
EDAT- 2007/07/20 09:00
MHDA- 2007/08/24 09:00
CRDT- 2007/07/20 09:00
PHST- 2007/07/20 09:00 [pubmed]
PHST- 2007/08/24 09:00 [medline]
PHST- 2007/07/20 09:00 [entrez]
AID - U3UN662552383654 [pii]
AID - 10.1309/21TN2RUWKR827UW2 [doi]
PST - ppublish
SO  - Am J Clin Pathol. 2007 Aug;128(2):323-32. doi: 10.1309/21TN2RUWKR827UW2.