PMID- 1763893
OWN - NLM
STAT- MEDLINE
DCOM- 19920212
LR  - 20071114
IS  - 0364-5134 (Print)
IS  - 0364-5134 (Linking)
VI  - 30
IP  - 5
DP  - 1991 Nov
TI  - Expression patterns of beta-amyloid precursor protein (beta-APP) in neural and 
      nonneural human tissues from Alzheimer's disease and control subjects.
PG  - 686-93
AB  - Both neural and nonneural human tissues from patients with or without Alzheimer's 
      disease (AD) were surveyed to detect the presence of the beta-amyloid protein and 
      its precursors. This was accomplished using polyclonal and monoclonal antibodies 
      to epitopes in the 695 amino acid long beta-APP (i.e., beta-APP695), as well as 
      in related beta-APPs. Immunoreactivity in beta-APP in brain was prominent in 
      senile plaques, extraneuronal tangles, and neurons. Outside the brain, beta-APP 
      staining was seen in neurons and satellite glial cells of the dorsal root, 
      enteric and trigeminal ganglia, the adeno- and neurohypophysis, megakaryocytes, 
      and adrenal gland in samples from patients with AD and those without AD. Western 
      blots of neocortex revealed three major proteins with apparent molecular masses 
      of 105, 115, and 125 kDa in the insoluble membrane-associated fractions, while 
      two broad bands with a molecular weight centered at about 100 and 120 kDa were 
      detected in soluble fractions. In addition, the pituitary and adrenal glands as 
      well as cardiac muscle revealed prominent immunobands in membrane-associated 
      fractions. Notably, other nonneural tissues were devoid of beta-APP 
      immunoreactivity. Thus, the beta-APPs are detectable only in a limited number of 
      nonneural tissues. Taken together, these data suggest that beta-APPs produced in 
      the brain are sources of beta-APP peptides that accumulate as senile plaques in 
      AD.
FAU - Arai, H
AU  - Arai H
AD  - Department of Pathology and Laboratory Medicine, University of Pennsylvania 
      School of Medicine, Philadelphia 19104.
FAU - Lee, V M
AU  - Lee VM
FAU - Messinger, M L
AU  - Messinger ML
FAU - Greenberg, B D
AU  - Greenberg BD
FAU - Lowery, D E
AU  - Lowery DE
FAU - Trojanowski, J Q
AU  - Trojanowski JQ
LA  - eng
GR  - AG-09215/AG/NIA NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Ann Neurol
JT  - Annals of neurology
JID - 7707449
RN  - 0 (Amyloid beta-Protein Precursor)
RN  - 0 (Nerve Tissue Proteins)
SB  - IM
MH  - Alzheimer Disease/*metabolism/pathology
MH  - Amyloid beta-Protein Precursor/*biosynthesis
MH  - Animals
MH  - Brain/metabolism/pathology
MH  - Gene Expression
MH  - Humans
MH  - Mice
MH  - Mice, Inbred BALB C/immunology
MH  - Muscles/metabolism/pathology
MH  - Nerve Tissue Proteins/*biosynthesis
MH  - Nervous System Diseases/metabolism/pathology
MH  - Organ Specificity
MH  - Peripheral Nerves/metabolism/pathology
MH  - Viscera/metabolism/pathology
EDAT- 1991/11/01 00:00
MHDA- 1991/11/01 00:01
CRDT- 1991/11/01 00:00
PHST- 1991/11/01 00:00 [pubmed]
PHST- 1991/11/01 00:01 [medline]
PHST- 1991/11/01 00:00 [entrez]
AID - 10.1002/ana.410300509 [doi]
PST - ppublish
SO  - Ann Neurol. 1991 Nov;30(5):686-93. doi: 10.1002/ana.410300509.