PMID- 17640485
OWN - NLM
STAT- MEDLINE
DCOM- 20081118
LR  - 20070720
IS  - 1671-4083 (Print)
IS  - 1671-4083 (Linking)
VI  - 28
IP  - 8
DP  - 2007 Aug
TI  - Antitumor effects of chi-shen extract from Salvia miltiorrhiza and Paeoniae radix 
      on human hepatocellular carcinoma cells.
PG  - 1215-23
AB  - AIM: To investigate the antihepatocellular carcinoma effects of chi-shen extract 
      (CSE) from the water-soluble compounds of Salvia miltiorrhiza and Paeoniae radix. 
      METHODS: The effect of CSE on the growth of HepG2 cells (hepatocellular carcinoma 
      cell line) was studied by 3-(4,5)-2,5-diphenyltetrazolium bromide assay. 
      Apoptosis were detected through acridine orange (AO) and ethylene dibromide (EB) 
      staining and DNA fragmentation assay. The effect of CSE on the cell cycle of 
      HepG2 cells was studied by the propidium iodide staining method. The activation 
      of caspases-3, -8 and -9 was examined by immunoassay kits. The transcription of 
      the Bcl-2 family and p53 was detected by RT-PCR. RESULTS: Our data revealed that 
      CSE strongly induced HepG2 cell death in a dose- and time-dependent manner. 
      CSE-induced cell death was considered to be apoptotic by observing the typical 
      apoptotic morphological change by AO/EB staining and DNA fragmentation assay. The 
      induction of HepG2 cell death was caused by an induction of apoptosis for the 
      sub-G1 proportion increase, the downregulation of Bcl-2, the upregulation of Bax 
      and p53, and the activation of the caspases-3 and -9 pathways. CONCLUSION: These 
      results clearly demonstrated that CSE was able to inhibit the proliferation of 
      HepG2 cells and cause apoptosis. Moreover, the anticancer effects of CSE were 
      related to the Bcl-2 family pathway and the activation of caspases-3 and -9 in 
      HepG2 cells.
FAU - Hu, Sheng
AU  - Hu S
AD  - Institute of Materia Media, South-Central University for Nationalities, Wuhan, 
      China.
FAU - Chen, Shi-min
AU  - Chen SM
FAU - Li, Xiao-kuan
AU  - Li XK
FAU - Qin, Rui
AU  - Qin R
FAU - Mei, Zhi-nan
AU  - Mei ZN
LA  - eng
PT  - Duplicate Publication
PT  - Journal Article
PL  - United States
TA  - Acta Pharmacol Sin
JT  - Acta pharmacologica Sinica
JID - 100956087
RN  - 0 (Antineoplastic Agents, Phytogenic)
RN  - 0 (Plant Preparations)
RN  - 0 (bcl-2-Associated X Protein)
SB  - IM
CIN - Acta Pharmacol Sin. 2007 Oct;28(10):1705. PMID: 17977096
MH  - Antineoplastic Agents, Phytogenic/*isolation & purification/*pharmacology
MH  - Apoptosis/*drug effects
MH  - Carcinoma, Hepatocellular/drug therapy
MH  - Cell Cycle
MH  - Cell Line, Tumor
MH  - Cell Survival/drug effects
MH  - Genes, bcl-2
MH  - Genes, p53
MH  - Humans
MH  - Liver Neoplasms/drug therapy
MH  - Paeonia/*chemistry
MH  - Plant Preparations/*pharmacology
MH  - Salvia miltiorrhiza/*chemistry
MH  - Transcription, Genetic
MH  - bcl-2-Associated X Protein/genetics
EDAT- 2007/07/21 09:00
MHDA- 2008/11/19 09:00
CRDT- 2007/07/21 09:00
PHST- 2007/07/21 09:00 [pubmed]
PHST- 2008/11/19 09:00 [medline]
PHST- 2007/07/21 09:00 [entrez]
AID - 10.1111/j.1745-7254.2007.00606.x [doi]
PST - ppublish
SO  - Acta Pharmacol Sin. 2007 Aug;28(8):1215-23. doi: 
      10.1111/j.1745-7254.2007.00606.x.