PMID- 17641037
OWN - NLM
STAT- MEDLINE
DCOM- 20070917
LR  - 20220227
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 179
IP  - 3
DP  - 2007 Aug 1
TI  - Triggering of dendritic cell responses after exposure to activated, but not 
      resting, apoptotic PBMCs.
PG  - 1711-20
AB  - Dendritic cells (DCs) can be activated by signaling via pathogen receptors, by 
      interaction with activated T cells or by exposure to inflammatory mediators. 
      Clearance of apoptotic cells by DCs is generally considered a silent event that 
      is not associated with an inflammatory response. Necrotic cell death, in 
      contrast, leads to induction of inflammation. However, emerging data challenge 
      the view of apoptotic cells as inherently nonimmunogenic. In this study, we 
      report that the activation state of the apoptotic cell may determine whether the 
      exposed DC becomes activated and rendered proficient in Ag presentation. We show 
      that coculture with activated, but not resting, apoptotic PBMCs leads to 
      up-regulation of surface expression of the costimulatory molecules CD80, CD83, 
      and CD86 in human DCs as well as release of proinflammatory cytokines. 
      Furthermore, we show that DCs exposed to allogeneic, activated apoptotic PBMCs 
      induce proliferation and IFN-gamma production in autologous T cells. Together, 
      these findings show that activated apoptotic PBMCs per se provide an 
      activation/maturation signal to DCs, suggesting that activated apoptotic PBMCs 
      possess endogenous adjuvant properties.
FAU - Johansson, Ulrika
AU  - Johansson U
AD  - Department of Medicine, Center for Infectious Medicine, Karolinska Institutet, 
      Karolinska University Hospital Huddinge, Stockholm, Sweden. 
      ulrika.johansson@ki.se
FAU - Walther-Jallow, Lilian
AU  - Walther-Jallow L
FAU - Smed-Sorensen, Anna
AU  - Smed-Sorensen A
FAU - Spetz, Anna-Lena
AU  - Spetz AL
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Isoantigens)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Apoptosis/*immunology
MH  - Cell Differentiation/immunology
MH  - Cell Proliferation
MH  - Cell-Free System/immunology
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Dendritic Cells/cytology/*immunology/*metabolism
MH  - Humans
MH  - Interferon-gamma/biosynthesis
MH  - Isoantigens/immunology/metabolism
MH  - Leukocytes, Mononuclear/*cytology/*immunology/pathology
MH  - Monocytes/cytology/immunology
MH  - Necrosis
MH  - Phagocytosis/immunology
MH  - Resting Phase, Cell Cycle/*immunology
MH  - T-Lymphocyte Subsets/immunology/metabolism
EDAT- 2007/07/21 09:00
MHDA- 2007/09/18 09:00
CRDT- 2007/07/21 09:00
PHST- 2007/07/21 09:00 [pubmed]
PHST- 2007/09/18 09:00 [medline]
PHST- 2007/07/21 09:00 [entrez]
AID - 179/3/1711 [pii]
AID - 10.4049/jimmunol.179.3.1711 [doi]
PST - ppublish
SO  - J Immunol. 2007 Aug 1;179(3):1711-20. doi: 10.4049/jimmunol.179.3.1711.