PMID- 17641652
OWN - NLM
STAT- MEDLINE
DCOM- 20070914
LR  - 20220409
IS  - 1750-2799 (Electronic)
IS  - 1750-2799 (Linking)
VI  - 2
IP  - 7
DP  - 2007
TI  - Fluorescence in situ hybridization on vibratome sections of plant tissues.
PG  - 1831-8
AB  - This protocol describes the application of fluorescence in situ hybridization 
      (FISH) to three-dimensionally (3D) preserved tissue sections derived from intact 
      plant structures such as roots or florets. The method is based on the combination 
      of vibratome sectioning with confocal microscopy. The protocol provides an 
      excellent tool to investigate chromosome organization in plant nuclei in all cell 
      types and has been used on tissues of both monocot and dicot plant species. The 
      visualization of 3D well-preserved tissues means that cell types can be 
      confidently identified. For example, meiocytes can be clearly identified at all 
      stages of meiosis and can be imaged in the context of their surrounding maternal 
      tissue. FISH can be used to localize centromeres, telomeres, repetitive regions 
      as well as unique regions, and total genomic DNAs can be used as probes to 
      visualize chromosomes or chromosome segments. The method can be adapted to RNA 
      FISH and can be combined with immunofluorescence labeling. Once the desired plant 
      material is sectioned, which depends on the number of samples, the protocol that 
      we present here can be carried out within 3 d.
FAU - Prieto, Pilar
AU  - Prieto P
AD  - John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, UK. 
      152prarm@uco.es
FAU - Moore, Graham
AU  - Moore G
FAU - Shaw, Peter
AU  - Shaw P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Nat Protoc
JT  - Nature protocols
JID - 101284307
RN  - 0 (Indicators and Reagents)
SB  - IM
MH  - Chromosomes/ultrastructure
MH  - Humidity
MH  - In Situ Hybridization, Fluorescence
MH  - Indicators and Reagents
MH  - Meiosis
MH  - Microscopy, Confocal/methods
MH  - Plant Cells
MH  - Plant Structures/chemistry/genetics
MH  - Plants/chemistry/*genetics
EDAT- 2007/07/21 09:00
MHDA- 2007/09/15 09:00
CRDT- 2007/07/21 09:00
PHST- 2007/07/21 09:00 [pubmed]
PHST- 2007/09/15 09:00 [medline]
PHST- 2007/07/21 09:00 [entrez]
AID - nprot.2007.265 [pii]
AID - 10.1038/nprot.2007.265 [doi]
PST - ppublish
SO  - Nat Protoc. 2007;2(7):1831-8. doi: 10.1038/nprot.2007.265.