PMID- 17643093
OWN - NLM
STAT- MEDLINE
DCOM- 20071025
LR  - 20230210
IS  - 0893-3952 (Print)
IS  - 0893-3952 (Linking)
VI  - 20
IP  - 9
DP  - 2007 Sep
TI  - EGFR expression as an ancillary tool for diagnosing lung cancer in cytology 
      specimens.
PG  - 905-13
AB  - Lung cancer evolves in a multistep process, and its early detection portends a 
      better prognosis. Bronchial washings/brushings and fine-needle aspirations are 
      often used as early screening and cytological diagnosis of lung cancer. In some 
      cases, it is difficult to differentiate morphologically malignant from reactive 
      cells. Epidermal growth factor receptor (EGFR) is a transmembrane receptor 
      overexpressed in high percentage lung cancers, and contributes to tumor growth. 
      Assessing EGFR expression levels by fluorescence in situ hybridization (FISH) and 
      immunohistochemistry (IHC) may provide critical information of tumor marker 
      abnormalities, assist in the cytological diagnosis, and stratify patients for 
      EGFR inhibitor therapy. Fifty patients with bronchial washings/brushings or 
      fine-needle aspiration specimens, and corresponding histologically confirmed lung 
      biopsies, were studied for EGFR expression with FISH and IHC. Copy numbers of the 
      EGFR gene locus were analyzed with those of chromosome 7 by FISH. EGFR and FISH 
      results were compared to our FISH data with combined EGFR, c-myc, 5p15.2, and 
      chromosome 6 probes in selected cases. Cell blocks, if available, and tissue 
      biopsy sections were used for EGFR IHC. The intensity of IHC was scored, and 
      quantified. Only balanced aneuploidy of EGFR was identified by FISH. Gene 
      amplification was not detected. The chromosomal abnormalities of EGFR were often 
      accompanied by other chromosomal aneuploidies demonstrated in c-myc (8q24), 
      5p15.2 or 6p, indicating a general genomic instability. About half of the 
      specimens with confirmed malignancy showed EGFR balanced aneuploidy by FISH, and 
      gene copy number was not coupled with protein expression in many cases. The 
      benign or reactive cytology specimens confirmed by biopsies had high specificity 
      by FISH (96%) and IHC (88%). FISH and IHC analysis of EGFR, possibly along with 
      other tumor markers, may be a useful ancillary tool to classify difficult 
      cytology cases and inform clinicians arranging targeted chemotherapy.
FAU - Wei, Eric X
AU  - Wei EX
AD  - Department of Hematopathology, University of Texas, MD Anderson Cancer Center, 
      Houston, TX 77030, USA. ericxwei@yahoo.com
FAU - Anga, Amal A
AU  - Anga AA
FAU - Martin, Sherry S
AU  - Martin SS
FAU - Jackson, Joyce G
AU  - Jackson JG
FAU - Nordberg, Mary L
AU  - Nordberg ML
FAU - Herrera, Guillermo A
AU  - Herrera GA
FAU - Turbat-Herrera, Elba A
AU  - Turbat-Herrera EA
LA  - eng
PT  - Journal Article
DEP - 20070720
PL  - United States
TA  - Mod Pathol
JT  - Modern pathology : an official journal of the United States and Canadian Academy 
      of Pathology, Inc
JID - 8806605
RN  - 0 (Biomarkers, Tumor)
RN  - EC 2.7.10.1 (EGFR protein, human)
RN  - EC 2.7.10.1 (ErbB Receptors)
SB  - IM
MH  - Aneuploidy
MH  - *Biomarkers, Tumor/analysis/genetics
MH  - Biopsy, Fine-Needle
MH  - Bronchoalveolar Lavage Fluid/chemistry/cytology
MH  - Carcinoma, Non-Small-Cell Lung/chemistry/*diagnosis/genetics/pathology
MH  - Carcinoma, Small Cell/chemistry/*diagnosis/genetics/pathology
MH  - Chromosomes, Human, Pair 5
MH  - Chromosomes, Human, Pair 6
MH  - Chromosomes, Human, Pair 7
MH  - *ErbB Receptors/analysis/genetics
MH  - *Gene Expression Regulation, Neoplastic
MH  - Genes, myc
MH  - Genomic Instability
MH  - Humans
MH  - *Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Lung Neoplasms/chemistry/*diagnosis/genetics/pathology
MH  - Predictive Value of Tests
MH  - Prognosis
MH  - Reproducibility of Results
EDAT- 2007/07/24 09:00
MHDA- 2007/10/27 09:00
CRDT- 2007/07/24 09:00
PHST- 2007/07/24 09:00 [pubmed]
PHST- 2007/10/27 09:00 [medline]
PHST- 2007/07/24 09:00 [entrez]
AID - S0893-3952(22)03263-X [pii]
AID - 10.1038/modpathol.3800939 [doi]
PST - ppublish
SO  - Mod Pathol. 2007 Sep;20(9):905-13. doi: 10.1038/modpathol.3800939. Epub 2007 Jul 
      20.