PMID- 17644114
OWN - NLM
STAT- MEDLINE
DCOM- 20080321
LR  - 20131121
IS  - 0022-4804 (Print)
IS  - 0022-4804 (Linking)
VI  - 145
IP  - 1
DP  - 2008 Mar
TI  - The use of an in vivo model to study the effects of hyperhomocysteinaemia on 
      vascular function.
PG  - 13-8
AB  - BACKGROUND/AIMS: To use an in vivo rat model of hyperhomocysteinaemia (HHCy) to 
      study its impact on vascular function. METHODS: Twenty rats were fed either a 
      control or HHCy-inducing diet for 10 wk. The response of aortic rings to 
      contraction with phenylephrine, and relaxation to acetylcholine 
      (endothelium-dependant relaxation) or sodium nitroprusside 
      (endothelium-independent relaxation) was analyzed. The results were compared 
      using an analysis of variance (ANOVA). RESULTS: There was a significant elevation 
      of HCy in the treated group (20.5 versus 1.6 micromol/L, P = 0.004). There was no 
      significant difference between the two groups in blood pressure measurements 
      (ANOVA, P = 0.152). In a dose-dependant manner, phenylephrine elicited 
      significantly greater contraction in aorta taken from HHCy rats than that taken 
      from controls (ANOVA, P < 0.001), acetylcholine elicited significantly less 
      percentage relaxation in aorta taken from HHCy rats than from controls (ANOVA, P 
      = 0.003) and though sodium nitroprusside stimulated less percentage relaxation in 
      aorta taken from HHCy rats than controls, this did not reach significance (ANOVA, 
      P = 0.051). CONCLUSIONS: In diet induced hyperhomocysteinaemic rats, there is 
      enhanced vascular contraction in response to phenylephrine and impaired 
      endothelium-dependant relaxation in response to acetylcholine.
FAU - Hansrani, Monica
AU  - Hansrani M
AD  - Northern Vascular Unit, Freeman Hospital, Newcastle upon Tyne, United Kingdom.
FAU - Stansby, Gerard
AU  - Stansby G
LA  - eng
PT  - Journal Article
DEP - 20070720
PL  - United States
TA  - J Surg Res
JT  - The Journal of surgical research
JID - 0376340
RN  - 0 (Vasoconstrictor Agents)
RN  - 0 (Vasodilator Agents)
RN  - 169D1260KM (Nitroprusside)
RN  - 1WS297W6MV (Phenylephrine)
RN  - 935E97BOY8 (Folic Acid)
RN  - AE28F7PNPL (Methionine)
RN  - N9YNS0M02X (Acetylcholine)
SB  - IM
MH  - Acetylcholine/pharmacology
MH  - Animals
MH  - Aorta/*physiopathology
MH  - Blood Pressure/drug effects
MH  - Disease Models, Animal
MH  - Dose-Response Relationship, Drug
MH  - Endothelium, Vascular/drug effects/physiopathology
MH  - Folic Acid
MH  - Hyperhomocysteinemia/chemically induced/*physiopathology
MH  - Male
MH  - Methionine
MH  - Nitroprusside/pharmacology
MH  - Phenylephrine/pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Vasoconstriction/drug effects/*physiology
MH  - Vasoconstrictor Agents/pharmacology
MH  - Vasodilator Agents/pharmacology
EDAT- 2007/07/24 09:00
MHDA- 2008/03/22 09:00
CRDT- 2007/07/24 09:00
PHST- 2006/08/26 00:00 [received]
PHST- 2007/02/08 00:00 [revised]
PHST- 2007/02/11 00:00 [accepted]
PHST- 2007/07/24 09:00 [pubmed]
PHST- 2008/03/22 09:00 [medline]
PHST- 2007/07/24 09:00 [entrez]
AID - S0022-4804(07)00293-4 [pii]
AID - 10.1016/j.jss.2007.02.055 [doi]
PST - ppublish
SO  - J Surg Res. 2008 Mar;145(1):13-8. doi: 10.1016/j.jss.2007.02.055. Epub 2007 Jul 
      20.