PMID- 17645513
OWN - NLM
STAT- MEDLINE
DCOM- 20071121
LR  - 20131121
IS  - 0143-2885 (Print)
IS  - 0143-2885 (Linking)
VI  - 40
IP  - 9
DP  - 2007 Sep
TI  - Hyperpolarization-activated channels in trigeminal ganglia innervating healthy 
      and pulp-exposed teeth.
PG  - 715-21
AB  - AIM: To use immunocytochemistry for determining the expression of HCN1, HCN2 and 
      HCN3 (three subunits of the hyperpolarization-activated cyclic nucleotide-gated 
      current channel) in rodent trigeminal ganglia (TG) that innervate healthy teeth 
      and determine if expression of HCN subunits is increased in TG following pulp 
      exposure. METHODOLOGY: Pulps were exposed in right maxillary incisors of male 
      Sprague-Dawley rats. After fixation, TG were removed, cryostat sectioned, and 
      immunocytochemistry was utilized to study the expression of HCN1-3 subunits. 
      Immunoreactivity of individual neurons from the maxillary region of the TG was 
      determined with ImageJ software. Differences in the number immunopositive neurons 
      amongst groups were tested for statistical significance with either a Yates or 
      Pearson's chi-square or Fisher's exact probability tests depending on neuron 
      sample size. Differences in the intensity of immunoreactivity between groups were 
      tested for statistical significance with a Student's t-test. RESULTS: The 
      majority of TG neurons were immunopositive for HCN1-3. Moreover, statistically 
      significant increases in the number of TG neurons immunopositive for HCN1 and the 
      intensity of HCN1-3 immunoreactivity were observed within hours of exposing the 
      tooth pulp. CONCLUSIONS: HCN1-3 expression, as determined by immunocytochemistry, 
      is increased within hours after injury. Given that I(h) can facilitate neuronal 
      excitability, results of the current study suggest that antagonists to HCN1-3 
      subunits could work as analgesics in the alleviation of orofacial pain.
FAU - Wells, J E
AU  - Wells JE
AD  - School of Dental Medicine, Southern Illinois University, Alton, IL 62002, USA. 
      jwells@siue.edu
FAU - Rowland, K C
AU  - Rowland KC
FAU - Proctor, E K
AU  - Proctor EK
LA  - eng
PT  - Journal Article
DEP - 20070723
PL  - England
TA  - Int Endod J
JT  - International endodontic journal
JID - 8004996
RN  - 0 (Cyclic Nucleotide-Gated Cation Channels)
RN  - 0 (Hcn1 protein, rat)
RN  - 0 (Hcn2 protein, rat)
RN  - 0 (Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels)
RN  - 0 (Ion Channels)
RN  - 0 (Potassium Channels)
MH  - Animals
MH  - Cyclic Nucleotide-Gated Cation Channels/*analysis
MH  - Dental Pulp/*injuries/innervation
MH  - Dental Pulp Exposure/*metabolism
MH  - Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels
MH  - Ion Channels/*analysis
MH  - Male
MH  - Potassium Channels/*analysis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Trigeminal Ganglion/*cytology
EDAT- 2007/07/25 09:00
MHDA- 2007/12/06 09:00
CRDT- 2007/07/25 09:00
PHST- 2007/07/25 09:00 [pubmed]
PHST- 2007/12/06 09:00 [medline]
PHST- 2007/07/25 09:00 [entrez]
AID - IEJ1297 [pii]
AID - 10.1111/j.1365-2591.2007.01297.x [doi]
PST - ppublish
SO  - Int Endod J. 2007 Sep;40(9):715-21. doi: 10.1111/j.1365-2591.2007.01297.x. Epub 
      2007 Jul 23.