PMID- 17646152
OWN - NLM
STAT- MEDLINE
DCOM- 20090327
LR  - 20190923
IS  - 0253-9772 (Print)
IS  - 0253-9772 (Linking)
VI  - 29
IP  - 7
DP  - 2007 Jul
TI  - [Isolation and characterization of the centromeric BAC clones from different 
      genomes in genus Oryza].
PG  - 851-8
AB  - Centromeres play an important role in ensuring the correct segregation and 
      transmission of chromosome during mitosis and meiosis in eukaryotes. In this 
      research, we constructed five BAC libraries for diploid wild rice with different 
      genomes. Together with the technique of colony blot hybridization and 
      fluorescence in situ hybridization (FISH), centromere-related BAC clones were 
      screened and characterized from different genomes. Meanwhile, co-hybridization 
      was detected between these clones and the five genomes. The results from this 
      study demonstrated that: (1) there were centromere-specific satellite repeat in 
      Oryza officinalis (CC genome) and O. brachyantha (FF genome), respectively, and 
      centromere-specific CRR-related sequence was found in O. brachyantha; (2) 
      homology sequences of CentO and CRR of O. sativa (AA genome) were detected on all 
      centromeres of O. glaberrima (AA genome), O. punctata (BB genome) and O. 
      australiensis (EE genome); And (3) the two somatic chromosomes of O. officinalis 
      comprised of homology sequences of CentO satellites as revealed FISH analysis 
      probed with RCS2. Homology sequences of CRR of O. sativa were also detected on 
      all centromeres of O. officinalis. The results provided a foundation toward 
      cloning the centromeric sequences from different genomes of genus Oryza, studying 
      centromere organization and evolution of different genome, analyzing the 
      relationship between centromeric structure and function among different genome.
FAU - Yi, Chuan-Deng
AU  - Yi CD
AD  - Key Lab of Plant Functional Genomics, Ministry of Education, Yangzhou University, 
      Jiangsu Province Key Lab of Crop Genetics and Physiology, Yangzhou 225009, China. 
      crops@yzu.edu.cn
FAU - Gong, Zhi-Yun
AU  - Gong ZY
FAU - Liang, Guo-Hua
AU  - Liang GH
FAU - Wang, Fei-Hua
AU  - Wang FH
FAU - Tang, Shu-Zhu
AU  - Tang SZ
FAU - Gu, Ming-Hong
AU  - Gu MH
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Yi Chuan
JT  - Yi chuan = Hereditas
JID - 9436478
SB  - IM
MH  - Centromere/*genetics
MH  - Chromosomes, Artificial, Bacterial/*genetics
MH  - Cloning, Molecular
MH  - Genome, Plant/*genetics
MH  - Genomic Library
MH  - In Situ Hybridization, Fluorescence
MH  - Oryza/*genetics
EDAT- 2007/07/25 09:00
MHDA- 2009/03/28 09:00
CRDT- 2007/07/25 09:00
PHST- 2007/07/25 09:00 [pubmed]
PHST- 2009/03/28 09:00 [medline]
PHST- 2007/07/25 09:00 [entrez]
AID - 0253-9772(2007)07-851-08 [pii]
AID - 10.1360/yc-007-0851 [doi]
PST - ppublish
SO  - Yi Chuan. 2007 Jul;29(7):851-8. doi: 10.1360/yc-007-0851.